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PMID: 6258019 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Site-specific integration of an F' lac pro factor in the region of the replication origin (oriC) of E. coli.

Molecular & general genetics : MGG ·Vol. 179 ·No. 2 ·1980-00-00 ·Pages 437-46

LaVerne LS, Ray DS

Abstract

An episome, F128, which carries approximately 8 x 10(4) base pairs of chromosomal DNA homologous to the lac pro region of the E. coli chromosome, has been found to integrate into the oriC region of the chromosome in a site specific reaction. While the event appears to be recA-dependent, no homology between the episome and this region of the chromosome was detected. The Hfr strains formed result from the integration of intact F128 molecules. The structure of the Hfr strains generated has been determined and their transfer properties analyzed.

MeSH Terms
Base Sequence Chromosomes, Bacterial Conjugation, Genetic DNA Transposable Elements Escherichia coli/genetics F Factor Recombination, Genetic
Chemicals
DNA Transposable Elements
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
LaVerne L S
Ray D S
References (23)
23 references, click to expand
  1. Enumeration and identification of IS3 elements in Escherichia coli strains.
    J Bacteriol. 1979 Mar;137(3):1421-4 PMID: 374349
  2. Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I.
    J Mol Biol. 1977 Jun 15;113(1):237-51 PMID: 881736
  3. Formation of merodiploids in matings with a class of Rec- recipient strains of Escherichia coli K12.
    Proc Natl Acad Sci U S A. 1968 May;60(1):160-7 PMID: 4873517
  4. Characterization of the dnaA, gyrB and other genes in the dnaA region of the Escherichia coli chromosome on specialized transducing phages lambda tna.
    Mol Gen Genet. 1979 Sep;175(2):135-44 PMID: 160000
  5. Agarose slab-gel electrophoresis equipment.
    Anal Biochem. 1975 Sep;68(1):36-46 PMID: 1190452
  6. Probability of F integration and frequency of stable Hfr donors in F+ populations of Escherichia coli K-12.
    Genetics. 1969 Sep;63(1):27-38 PMID: 4903892
  7. CHROMOSOME TRANSFER IN BACTERIAL CONJUGATION.
    Bacteriol Rev. 1965 Jun;29:161-72 PMID: 14310189
  8. Studies with IS1 and IS4.
    Cold Spring Harb Symp Quant Biol. 1979;43 Pt 2:1187-92 PMID: 385222
  9. Electron microscopic heteroduplex studies of sequence relations among plasmids of Escherichia coli: structure of F13 and related F-primes.
    J Bacteriol. 1975 May;122(2):749-63 PMID: 1092667
  10. Chromosome replication in Escherichia coli. IV. Control of chromosome replication and cell division by an integrated episome.
    J Mol Biol. 1971 Feb 14;55(3):441-56 PMID: 4927942
  11. Multiple copies of the insertion-DNA sequences IS1 and IS2 in the chromosome of E. coli K-12.
    Mol Gen Genet. 1973 May 9;122(3):267-77 PMID: 4577900
  12. Escherichia coli mutants temperature-sensitive for DNA synthesis.
    Mol Gen Genet. 1971;113(3):273-84 PMID: 4946856
  13. Detection of specific sequences among DNA fragments separated by gel electrophoresis.
    J Mol Biol. 1975 Nov 5;98(3):503-17 PMID: 1195397
  14. Studies on the cleavage of bacteriophage lambda DNA with EcoRI Restriction endonuclease.
    J Mol Biol. 1975 Jan 25;91(3):315-28 PMID: 1102702
  15. Two kinds of insertions in bacterial genes.
    Mol Gen Genet. 1972;119(3):191-206 PMID: 4567154
  16. Escherichia coli K-12 F-prime factors, old and new.
    Bacteriol Rev. 1972 Dec;36(4):587-607 PMID: 4568765
  17. Origin of replication, oriC, of the Escherichia coli chromosome: mapping of genes relative to R.EcoRI cleavage sites in the oriC region.
    Mol Gen Genet. 1977 Dec 14;158(1):101-9 PMID: 342904
  18. Electron microscope heteroduplex studies of sequence relations among plasmids of Escherichia coli. I. Structure of F-prime factors.
    J Mol Biol. 1972 Nov 14;71(2):471-97 PMID: 4564488
  19. Recalibrated linkage map of Escherichia coli K-12.
    Bacteriol Rev. 1976 Mar;40(1):116-67 PMID: 773363
  20. Map location of the Escherichia coli origin of replication.
    Mol Gen Genet. 1978 Nov 9;166(3):299-304 PMID: 368578
  21. Suppression of initiation-negative strains of Escherichia coli by integration of the sex factor F.
    J Bacteriol. 1975 Feb;121(2):554-61 PMID: 1089635
  22. Electron microscope heteroduplex studies of sequence relations among plasmids of Escherichia coli. IV. The F sequences in F14.
    J Mol Biol. 1974 Nov 15;89(4):565-84 PMID: 4615159
  23. Bacteriophage lambda carrying the Escherichia coli chromosomal region of the replication origin.
    Proc Natl Acad Sci U S A. 1978 Oct;75(10):5099-103 PMID: 368808
Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00425474
Published
1980-00-00
Pages
437-46
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00425474
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