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PMID: 6251453 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

5,6-dichloro-1-beta-ribofuranosylbenzimidazole enhances premature termination of late transcription of simian virus 40 DNA.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 77 ·No. 6 ·1980-06-00 ·Pages 3297-3301

Laub O, Jakobovits EB, Aloni Y

Abstract

Short RNA chains initiating at the major promoter sites for simian virus 40 (SV40) late transcription are elongated to approximately 450 nucleotides in a molar ammount greater than that from any other region of the viral DNA. This conclusion is based on the following observations: (i) Transcriptional complexes isolated by Sarkosyl and by hypotonic leaching (minichromosomes) from nuclei of cells infected with SV40 as well as intact nuclei were pulse labeled in vitro with [alpha-32P]TUP and were observed to synthesize short RNA transcripts that hybridized predominantly to a SV40 DNA fragment spanning between 0.67 and 0.76 map units. (ii) In the presence of 5,6-dichloro-1-beta-D-ribofuranosylbenzimidazole (DRB), a drug known to accentuate premature transcriptional termination, accumulation of these short SV40 RNA chains was enhanced. When SV40-infected cells were pretreated with DRB and then labeled in vivo or in vitro, they synthesized short labeled viral RNAs that hydridized almost exclusively with the DNA fragment spanning between 0.67 and 0.76 map units. These observations suggest a mechanism in the regulation of SV40 late transcription.

MeSH Terms
Animals Cells, Cultured DNA, Viral/metabolism Dichlororibofuranosylbenzimidazole/pharmacology Haplorhini Operon RNA, Viral/biosynthesis Ribonucleosides/pharmacology Simian virus 40/genetics,metabolism Time Factors Transcription, Genetic/drug effects
Chemicals
DNA, Viral RNA, Viral Ribonucleosides Dichlororibofuranosylbenzimidazole
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Laub O
Jakobovits E B
Aloni Y
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40 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.77.6.3297
Published
1980-06-00
Pages
3297-3301
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC349602
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.77.6.3297 PMC Full Text View journal details
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