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PMID: 6246439 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

The lambda phage att site: functional limits and interaction with Int protein.

Nature ·Vol. 285 ·No. 5760 ·1980-05-08 ·Pages 85-91

Hsu PL, Ross W, Landy A

Abstract

Site specific integrative recombination of bacteriophage lambda involves unequal partners. The minimal phage att site is composed of approximately 240-base pairs and four distinct binding sites for Int protein, at least three of which are crucial for function. This 'donor site' recombines efficiently with a smaller 'recipient site' that lacks the extensive interactions with Int protein.

MeSH Terms
Attachment Sites, Microbiological Bacteriophage lambda/genetics Base Sequence DNA Helicases/genetics DNA, Bacterial/genetics DNA, Superhelical/genetics DNA, Viral/genetics Escherichia coli/genetics Integrases Lysogeny Plasmids Viral Proteins/physiology
Chemicals
DNA, Bacterial DNA, Superhelical DNA, Viral Viral Proteins Integrases DNA Helicases
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Hsu P L
Ross W
Landy A
References (25)
25 references, click to expand
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Article Info
Journal
Nature
Abbr.
Nature
ISSN
0028-0836
DOI
10.1038/285085a0
Published
1980-05-08
Pages
85-91
Language
English
Region
England
NLM ID
0410462
PMCID
PMC1994824
Subset
IM
Grants
NIGMS NIH HHS · R01 GM033928 · United States
NIGMS NIH HHS · R01 GM062723 · United States
Databases
GENBANK
J02459, M17233
External Links
PubMed source DOI: 10.1038/285085a0 PMC Full Text View journal details
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