Repair of x-ray-induced deoxyribonucleic acid single-strand breaks in xth mutants of Escherichia coli.

Journal of bacteriology ·Vol. 141 ·No. 3 ·1980-03-00 ·Pages 1424-7

Seeberg E, Steinum AL

Abstract

An exonuclease III-deficient strain of Escherichia coli K-12, BW2001 (xthA11), was unable to perform rapid repair of X-ray-induced deoxyribonucleic acid single-strand breaks and appeared to have a defect in the priming of the 3'-termini necessary for initiation of repair synthesis at the breaks. This defect cannot be explained solely by the lack of exonuclease III activity, because other xth mutants tested, including a deletion mutant, repaired radiation-induced strand breaks at close to the normal rate.

MeSH Terms

DNA Repair DNA, Bacterial/metabolism,radiation effects DNA, Single-Stranded/metabolism Escherichia coli/genetics,metabolism Exonucleases/metabolism Mutation X-Rays

Chemicals

DNA, Bacterial DNA, Single-Stranded Exonucleases

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Seeberg E

Steinum A L

References (15)

15 references, click to expand

Properties of a deoxyribonucleic acid ligase mutant of Escherichia coli: x-ray sensitivity.
J Bacteriol. 1970 May;102(2):588-9 PMID: 4911548
Genetic and molecular characteristics of X-ray-sensitive mutants of Escherichia coli defective in repair synthesis.
J Bacteriol. 1970 Nov;104(2):871-81 PMID: 4923076
DNA polymerase required for rapid repair of x-ray--induced DNA strand breaks in vivo.
Science. 1971 May 21;172(3985):851-4 PMID: 4929534
Replication and repair of DNA in cells of Escherichia coli treated with toluene.
Proc Natl Acad Sci U S A. 1970 Oct;67(2):674-81 PMID: 4943178
The formation of single-strand breaks in intracellular DNA by x-rays.
Radiat Res. 1971 Dec;48(3):599-612 PMID: 4944777
In vitro repair of x-irradiated DNA extracted from Bacillus subtilis deficient in polymerase I.
Proc Natl Acad Sci U S A. 1972 Nov;69(11):3211-4 PMID: 4628873
X-ray sensitivity and repair capacity of a polA1 exrA strain of Escherichia coli K-12.
J Bacteriol. 1973 Apr;114(1):121-7 PMID: 4572706
The rapair of DNA single-strand breaks in E. coli K-12 x-irradiated in the presence or absence of oxygen; the influence of repair on cell survival.
Radiat Res. 1973 Aug;55(2):334-45 PMID: 4579017
Mutations simultaneously affecting endonuclease II and exonuclease III in Escherichia coli.
Proc Natl Acad Sci U S A. 1975 Feb;72(2):688-92 PMID: 1091930
Role for deoxyribonucleic acid ligase in deoxyribonucleic acid polymerase i-dependent repair synthesis in toluene-treated escherichia coli.
J Bacteriol. 1975 Oct;124(1):585-8 PMID: 170252
Excision repair of ultraviolet-irradiated deoxyribonucleic acid in plasmolyzed cells of Escherichia coli.
J Bacteriol. 1976 Mar;125(3):787-95 PMID: 767327
Methyl methane sulfonate-sensitive mutant of Escherichia coli deficient in an endonuclease specific for apurinic sites in deoxyribonucleic acid.
J Bacteriol. 1976 May;126(2):646-53 PMID: 177402
Role of deoxyribonucleic acid polymerases and deoxyribonucleic acid ligase in x-ray-induced repair synthesis in toluene-treated Escherichia coli K-12.
J Bacteriol. 1976 May;126(2):785-93 PMID: 177404
In vitro repair of radiation-induced strand breaks in DNA.
Biochim Biophys Acta. 1976 May 3;432(2):145-53 PMID: 773432
Genetic mapping of xthA, the structural gene for exonuclease III in Escherichia coli K-12.
J Bacteriol. 1976 Jun;126(3):1082-8 PMID: 780339

Article Info

Journal

Journal of bacteriology

Abbr.

J Bacteriol

ISSN

0021-9193

DOI

10.1128/jb.141.3.1424-1427.1980

Published

1980-03-00

Pages

1424-7

Language

English

Region

United States

NLM ID

2985120R

PMCID

PMC293841

Subset

External Links

PubMed source DOI: 10.1128/jb.141.3.1424-1427.1980 PMC Full Text View journal details

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Repair of x-ray-induced deoxyribonucleic acid single-strand breaks in xth mutants of Escherichia coli.

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