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PMID: 6234883 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Identification and characterization of Ca2+-phospholipid-dependent protein kinase in rat islets and hamster beta-cells.

The Biochemical journal ·Vol. 219 ·No. 2 ·1984-04-15 ·Pages 547-51

Lord JM, Ashcroft SJ

Abstract

We show that extracts of rat islets of Langerhans and of cloned hamster beta-cells (HIT-T15 cells) contain Ca2+-phospholipid-dependent protein kinase and endogenous protein substrates for the kinase. We purified Ca2+-phospholipid-dependent protein kinase from HIT-T15 beta-cells and report here its physical and kinetic properties.

MeSH Terms
Animals Calcium/pharmacology Cell Line Chromatography, Gel Cricetinae Islets of Langerhans/enzymology Kinetics Phospholipids/pharmacology Phosphorylation Protein Kinase C Protein Kinases/isolation & purification Rats Rats, Inbred Strains
Chemicals
Phospholipids Protein Kinases Protein Kinase C Calcium
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Lord J M
Ashcroft S J
References (29)
29 references, click to expand
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Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj2190547
Published
1984-04-15
Pages
547-51
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1153512
Subset
IM
External Links
PubMed source DOI: 10.1042/bj2190547 PMC Full Text View journal details
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