The radial distribution of succinate dehydrogenase activity in porcine muscle fibres.

The Histochemical journal ·Vol. 16 ·No. 3 ·1984-03-00 ·Pages 321-9

Swatland HJ

Abstract

A microscope photometer with a computer-controlled scanning stage was used to map the distribution of succinate dehydrogenase (SDH) activity in transverse sections of skeletal muscle fibres of pigs from 9 to 29 weeks of age. Absorbance due to SDH activity was measured in successive concentric zones that converged on the central axis of the muscle fibre. In all fibres, there was less SDH activity in the axis of the fibre than in the periphery of the fibre. In fibres with strong adenosine triphosphatase (ATPase) activity and weak overall SDH activity, the radial gradient of SDH activity remained constant as fibres grew in cross-sectional area. However, in fibres with weak ATPase and strong SDH and in fibres with strong ATPase and strong SDH, the radial gradient of SDH activity increased as fibres grew larger. Changes in radial gradients were due to both decreased SDH activity in the axis and to increased SDH activity in the periphery. In all three fibre types, the overall SDH activity per fibre increased with age.

MeSH Terms

Adenosine Triphosphatases/metabolism Aging Animals Female Muscle Development Muscles/enzymology Photometry Succinate Dehydrogenase/metabolism Swine/growth & development,metabolism

Chemicals

Succinate Dehydrogenase Adenosine Triphosphatases

Authors & Affiliations

1 authors, click to expand affiliations / ORCID

Swatland H J

References (9)

9 references, click to expand

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Article Info

Journal

The Histochemical journal

Abbr.

Histochem J

ISSN

0018-2214

DOI

10.1007/BF01003615

Published

1984-03-00

Pages

321-9

Language

English

Region

Netherlands

NLM ID

0163161

Subset

External Links

PubMed source DOI: 10.1007/BF01003615

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The radial distribution of succinate dehydrogenase activity in porcine muscle fibres.

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