The quantification of formazans in tissue sections by microdensitometry. I. The use of neotetrazolium chloride.

The Histochemical journal ·Vol. 8 ·No. 4 ·1976-07-00 ·Pages 373-81

Altman FP

Abstract

This article describes the use of a microdensitometer for the measurement of formazan deposits in tissue sections. Some examples are given to illustrate the various applications of this technique in the assessment of glucose-6-phosphate dehydrogenase activity. These are (I) the separate measurement of the red half-formazan intermediate and purple diformazan of neotetrazolium, and the effect of incubation time on their production, (2) the measurement of activities in different regions of the liver lobule, and the selective effect of phenobarbitone, and (3) the measurement of enzyme activity in individual cartilage cells in normal and osteoarthrosis-prone animals. All activities can be expressed in absolute units as nmol hydrogen/mm3/hr, and thus compared with standard biochemical data. The activities obtained all fall within the range of published values for biochemical systems.

MeSH Terms

Animals Azo Compounds Cartilage/enzymology Densitometry/methods Female Formazans Glucosephosphate Dehydrogenase/metabolism Kinetics Liver/cytology,enzymology Male Mice Microchemistry Osteoarthritis/enzymology Rats Staining and Labeling Tetrazolium Salts

Chemicals

Azo Compounds Formazans Tetrazolium Salts Glucosephosphate Dehydrogenase

Authors & Affiliations

1 authors, click to expand affiliations / ORCID

Altman F P

References (16)

16 references, click to expand

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Article Info

Journal

The Histochemical journal

Abbr.

Histochem J

ISSN

0018-2214

DOI

10.1007/BF01003826

Published

1976-07-00

Pages

373-81

Language

English

Region

Netherlands

NLM ID

0163161

Subset

External Links

PubMed source DOI: 10.1007/BF01003826

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The quantification of formazans in tissue sections by microdensitometry. I. The use of neotetrazolium chloride.

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