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PMID: 6223021 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Two fep genes are required for ferrienterochelin uptake in Escherichia coli K-12.

Journal of bacteriology ·Vol. 155 ·No. 1 ·1983-07-00 ·Pages 330-6

Pierce JR, Pickett CL, Earhart CF

Abstract

Escherichia coli mutants defective in the assimilation of iron from ferrienterochelin were isolated and characterized. One mutant was able to bind ferrienterochelin to its outer membrane but could not transport it into the cell. Complementation tests with lambda hybrid phage were employed to distinguish the defective gene, which we term fepB, from fepA, the structural gene for the outer membrane ferrienterochelin receptor protein. These same physiological and genetic tests were employed to tentatively classify several previously described fep mutants as carrying either fepA or fepB. The data demonstrate the existence of fepB and provide an explanation for previous difficulties in identifying fepB mutants.

MeSH Terms
Cell Membrane/metabolism Enterobactin/genetics,metabolism Escherichia coli/genetics Genes Genes, Bacterial Genetic Linkage Iron/metabolism Kinetics Mutation Serine/analogs & derivatives Species Specificity
Chemicals
Enterobactin Serine ferrienterochelin Iron
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Pierce J R
Pickett C L
Earhart C F
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31 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.155.1.330-336.1983
Published
1983-07-00
Pages
330-6
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC217684
Subset
IM
Grants
NIAID NIH HHS · AI 17794 · United States
External Links
PubMed source DOI: 10.1128/jb.155.1.330-336.1983 PMC Full Text View journal details
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