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PMID: 6223012 Published · ppublish English Comparative Study Journal Article

Differentiation of fiber types in skeletal muscle from the sequential inactivation of myofibrillar actomyosin ATPase during acid preincubation.

Histochemistry ·Vol. 77 ·No. 4 ·1983-00-00 ·Pages 543-55

Gollnick PD, Parsons D, Oakley CR

Abstract

A method is described for identifying fiber types of skeletal muscle from several mammalian species on the basis of the sequential inactivation of myofibrillar actomyosin ATPase during acid preincubation. When this method is used in combination with the standard alkaline preincubation at least 5 types of fibers can be identified. Of these, 2 are type I fibers with those of the slow twitch soleus muscle being different from those that exist in mixed muscles. The 3 subtypes of type II fibers exist independent of their metabolic properties. The need for careful standardization of histochemical methods for the visualization of myofibrillar actomyosin ATPase and the implication of the existence of different fiber types in apparently homogeneous muscle for the preparation of antibodies used for immunocytochemical methods of fiber identification are discussed.

MeSH Terms
Adenosine Triphosphatases/antagonists & inhibitors Animals Dogs Histocytochemistry Horses Hydrogen-Ion Concentration Kinetics Muscles/enzymology Myofibrils/enzymology Organ Specificity Rabbits Rats Species Specificity
Chemicals
Adenosine Triphosphatases
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Gollnick P D
Parsons D
Oakley C R
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35 references, click to expand
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Article Info
Journal
Histochemistry
Abbr.
Histochemistry
ISSN
0301-5564
DOI
10.1007/BF00495808
Published
1983-00-00
Pages
543-55
Language
English
Region
Germany
NLM ID
0411300
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00495808
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