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PMID: 144718 Published · ppublish English Journal Article

Myosin light chain patterns of individual fast and slow-twitch fibres of rabbit muscles.

Histochemistry ·Vol. 54 ·No. 2 ·1977-10-22 ·Pages 97-107

Pette D, Schnez U

Abstract

Single muscle fibres were isolated by microdissection from freeze-dried samples of rabbit psoas and soleus muscles. The individual fibres were typed according to qualitative histochemical reactions for succinate dehydrogenase or NADH-tetrazolium reductase and for alkaline Ca2+-activated myofibrilla myosin ATPase after acid or alkaline preincubation. Methods are described for electrophoretic analysis by means of polyacrylamide disc electrophoresis in the presence of SDS of total myofibrilla proteins in single fibres after pre-extraction of soluble proteins. Fast-twitch white fibres revealed a myosin light chain pattern characteristic of "fast- type" myosin with three light chains of apparent molecular weights of 22,300 (LC1) 18,400 (LC2) and 16,000 (LC3). Fast-twitch red fibres were indistinguishable in this respect from fast-twist white fibres and showed an identical pattern of myosin light chains. Slow-twitch fibres could be characterized by a myosin light chain pattern typical of myosin of slow-twitch muscles with peptides of the apparent molecular weights of 23,500 (LC1Sa), 23,000 (LC1Sb) and 18,500 (LS2S). Slow-twitch fibres isolated from soleus as well as from psoas muscle were indistinguishable with regard to their myosin light chain patterns, thus suggesting that fibres of the same histochemical type correspond in their myosin light chain patterns irrespective of their origin from different muscles.

MeSH Terms
Adenosine Triphosphatases/metabolism Animals Calcium/pharmacology Histocytochemistry Molecular Weight Muscle Proteins/analysis Muscles/enzymology Myosins Peptide Fragments Rabbits Succinate Dehydrogenase/metabolism
Chemicals
Muscle Proteins Peptide Fragments Succinate Dehydrogenase Adenosine Triphosphatases Myosins Calcium
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Pette D
Schnez U
References (32)
32 references, click to expand
  1. Metabolic characteristics of fibre types in human skeletal muscle.
    Acta Physiol Scand. 1975 Oct;95(2):153-65 PMID: 242187
  2. Dynamic properties of mammalian skeletal muscles.
    Physiol Rev. 1972 Jan;52(1):129-97 PMID: 4256989
  3. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
    Nature. 1970 Aug 15;227(5259):680-5 PMID: 5432063
  4. The specificity of the histochemical method for adenosine triphosphatase.
    J Histochem Cytochem. 1955 May;3(3):170-95 PMID: 14381606
  5. Histochemical composition, contraction speed and fatiguability of rat soleus motor units.
    J Neurol Sci. 1973 Oct;20(2):177-98 PMID: 4270833
  6. Qualitative differences between actomyosin ATPase of slow and fast mammalian muscle.
    Exp Neurol. 1969 Sep;25(1):138-52 PMID: 4241609
  7. Enzyme histochemical studies on ATPase activities in longitudinal sections of striated muscle tissue in the rat.
    Histochem J. 1973 May;5(3):265-9 PMID: 4270160
  8. Characterization of myosin light chains from histochemically identified fibres of rabbit psoas muscle.
    FEBS Lett. 1975 Jan 1;49(3):320-4 PMID: 122817
  9. Time dependent effects on contractile properties, fibre population, myosin light chains and enzymes of energy metabolism in intermittently and continuously stimulated fast twitch muscles of the rabbit.
    Pflugers Arch. 1976 Jul 30;364(2):103-12 PMID: 134352
  10. The content of troponin, tropomyosin, actin, and myosin in rabbit skeletal muscle myofibrils.
    Arch Biochem Biophys. 1974 Jun;162(2):436-41 PMID: 4407361
  11. MYOSIN OF FAST AND SLOW MUSCLES OF THE RABBIT.
    Arch Biochem Biophys. 1965 Jan;109 :185-91 PMID: 14281944
  12. ATPase activity of myosin correlated with speed of muscle shortening.
    J Gen Physiol. 1967 Jul;50(6):Suppl:197-218 PMID: 4227924
  13. Muscle fiber types: how many and what kind?
    Arch Neurol. 1970 Oct;23(4):369-79 PMID: 4248905
  14. Metabolic profiles of three fiber types of skeletal muscle in guinea pigs and rabbits.
    Biochemistry. 1972 Jul 4;11(14):2627-33 PMID: 4261555
  15. Hindlimb muscle fiber populations of five mammals.
    J Histochem Cytochem. 1973 Jan;21(1):51-5 PMID: 4348494
  16. Activity patterns of phosphofructokinase, glyceraldehydephosphate dehydrogenase, lactate dehydrogenase and malate dehydrogenase in microdissected fast and slow fibres from rabbit psoas and soleus muscle.
    Histochemistry. 1977 Jun 8;52(3):201-16 PMID: 142072
  17. An electrophoretic study of the low-molecular-weight components of myosin.
    Biochem J. 1970 Aug;119(1):31-8 PMID: 5485752
  18. ATPase activities, Ca2+ transport and phosphoprotein formation in sarcoplasmic reticulum subfractions of fast and slow rabbit muscles.
    Eur J Biochem. 1977 Dec 1;81(2):211-22 PMID: 145941
  19. Myosin from cross-reinnervated cat muscles.
    Nature. 1974 Jan 18;247(5437):135-9 PMID: 4810223
  20. The myosin of rabbit red muscles.
    Arch Biochem Biophys. 1968 Sep 20;127(1):370-5 PMID: 4235224
  21. ENZYMES IN MUSCLE. I. HISTOCHEMICAL STUDIES OF ENZYMES IN INDIVIDUAL MUSCLE FIBERS.
    Arch Neurol. 1964 Oct;11:355-8 PMID: 14196728
  22. Letter to the editor: Subtypes of the histochemical type I muscle fibers.
    J Histochem Cytochem. 1975 Jan;23(1):89-91 PMID: 46875
  23. Light chains from fast and slow muscle myosins.
    Nature. 1971 Nov 12;234(5324):81-5 PMID: 4942892
  24. Studies on myosin from red and white skeletal muscles of the rabbit. I. Adenosine triphosphatase activity.
    J Biol Chem. 1966 Dec 25;241(24):5772-6 PMID: 4224728
  25. Comparative studies on myofibrillar proteins in different types of skeletal muscle fibers.
    Exp Neurol. 1972 Dec;37(3):515-21 PMID: 4650892
  26. Histochemical, biochemical, and contractile properties of red, white, and intermediate fibers.
    Am J Physiol. 1971 Feb;220(2):410-4 PMID: 4250606
  27. Histochemical characteristics of vertebrate striated muscle: a review.
    Prog Histochem Cytochem. 1976;8(4):1-48 PMID: 185661
  28. Further histochemical properties of rabbit skeletal muscle fibres.
    Histochemie. 1973;36(2):173-83 PMID: 4269553
  29. Differences in histochemical attributes between diaphragm and hindleg muscles of the rat.
    Anat Rec. 1972 Jul;173(3):333-9 PMID: 4114182
  30. Differences between slow and fast muscle myosin. Adenosine triphosphatase activity and release of associated proteins by p-chloromercuriphenylsulfonate.
    J Biol Chem. 1970 Jan 25;245(2):219-24 PMID: 4243949
  31. Chemical studies on light chains from cardiac and skeletal muscle myosins.
    Nature. 1971 Nov 12;234(5324):85-8 PMID: 4942893
  32. Light chains of myosins from white, red, and cardiac muscles.
    Proc Natl Acad Sci U S A. 1971 May;68(5):946-50 PMID: 4252540
Article Info
Journal
Histochemistry
Abbr.
Histochemistry
ISSN
0301-5564
DOI
10.1007/BF00489668
Published
1977-10-22
Pages
97-107
Language
English
Region
Germany
NLM ID
0411300
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00489668
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