The nucleotide sequence at the 3'-end of Neurospora crassa 18S-rRNA and studies on the interaction with 5S-rRNA.

Nucleic acids research ·Vol. 10 ·No. 21 ·1982-11-11 ·Pages 6733-45

Kelly JM, Cox RA

Abstract

The sequence of more than 100 nucleotides at the 3'-end of Neurospora crassa 18S-rRNA was determined by chemical sequencing techniques. Extensive homologies with 18S-rRNA from other eukaryotes were found. Inspection of the nucleotide sequence at the 3'-end of N. crassa 5S-rRNA revealed the presence of sequences complementary to a region near the 3'-terminus of 18S-rRNA. Under the appropriate conditions a complex was formed between 18S-rRNA and 5S-rRNA (Tm 53 degrees C). Interaction was detected between 5S-rRNA and a specific 3'-terminal fragment from 18S-rRNA and between 18S-rRNA and a specific 3'-terminal fragment from 5S-rRNA. These findings are consistent with the idea that intermolecular base-pairing between nucleotides at the 3'-ends of 18S-rRNA and 5S-rRNA may be functionally important within the ribosome. Further investigation revealed that this intermolecular base-pairing is not essential for ribosome stability.

MeSH Terms

Animals Base Sequence Molecular Weight Neurospora/genetics Neurospora crassa/genetics Nucleic Acid Conformation Polyribosomes/metabolism Protein Biosynthesis RNA, Ribosomal/genetics Rabbits Reticulocytes/metabolism Species Specificity

Chemicals

RNA, Ribosomal

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Kelly J M

Cox R A

References (23)

23 references, click to expand

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Article Info

Journal

Nucleic acids research

Abbr.

Nucleic Acids Res

ISSN

0305-1048

DOI

10.1093/nar/10.21.6733

Published

1982-11-11

Pages

6733-45

Language

English

Region

England

NLM ID

0411011

PMCID

PMC326960

Subset

Databases

GENBANK

M11033

External Links

PubMed source DOI: 10.1093/nar/10.21.6733 PMC Full Text View journal details

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The nucleotide sequence at the 3'-end of Neurospora crassa 18S-rRNA and studies on the interaction with 5S-rRNA.

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