Identification of a protein of 15,000 daltons related to isoleucine-valine biosynthesis in Escherichia coli K-12.
The effect of the ilvG671, ilvG468, and ilvG603 mutations (phenotype, IlvG+ Valr; formerly ilvO) upon proteins synthesized was determined by infection of irradiated Escherichia coli K-12 cells, using specifically constructed derivatives of lambda dilv phage. These ilvG alleles are similar to the previously studied ilvG2096(Valr) allele in that they activate the latent ilvG gene which is present in the wild-type strain, leading to the synthesis of a 62,000-dalton protein. In addition, all of these ilvG (Valr) alleles increase the synthesis of a 15,000-dalton protein. To localize the gene coding for the 15,000-dalton protein, the proteins produced in maxicells containing plasmids with specific deletions of ilv and rrnX DNA segments were analyzed. The gene coding for the 15,000-dalton protein was located within a region about 1,000 base pairs long between ilv and trpT. The function of the 15,000-dalton protein is not known.
28 references, click to expand
-
Specific binding of leucyl transfer RNA to an immature form of L-threonine deaminase: its implications in repression.Proc Natl Acad Sci U S A. 1970 Aug;66(4):1027-35 PMID: 4920088
-
Expression of a valine-resistant acetolactate synthase activity mediated by the ilv O and ilv G genes of Escherichia coli K-12.Mol Gen Genet. 1976 Feb 2;143(3):243-52 PMID: 765758
-
The regulation of the ilv ADGE operon: evidence for positive control by threonine deaminase.J Mol Biol. 1976 Apr 15;102(3):453-65 PMID: 775108
-
Specialized transducing phage for the initiation factor 3 gene in Escherichia coli.Proc Natl Acad Sci U S A. 1977 Sep;74(9):3970-4 PMID: 333453
-
Computer analysis of nucleic acid regulatory sequences.Proc Natl Acad Sci U S A. 1977 Oct;74(10):4401-5 PMID: 270683
-
Genetics of bacterial ribosomes.Annu Rev Genet. 1977;11:297-347 PMID: 339818
-
Construction and characterization of new cloning vehicles. II. A multipurpose cloning system.Gene. 1977;2(2):95-113 PMID: 344137
-
Dual autogenous regulatory role of threonine deaminase in Escherichia coli K-12.Mol Gen Genet. 1978 Feb 7;159(1):27-32 PMID: 345081
-
Amino acid biosynthesis and its regulation.Annu Rev Biochem. 1978;47:532-606 PMID: 354503
-
Regulation of isoleucine and valine biosynthesis.Curr Top Cell Regul. 1978;14:29-73 PMID: 365469
-
A site of action for tRNA mediated regulation of the ilvOEDA operon of Escherichia coli K12.Mol Gen Genet. 1978 Nov 29;167(2):227-34 PMID: 366386
-
Deletion analysis of the expression of rRNA genes and associated tRNA genes carried by a lambda transducing bacteriophage.J Bacteriol. 1979 Jan;137(1):507-16 PMID: 368028
-
Deletion mapping of the ilvGOEDAC genes of Escherichia coli K-12.Mol Gen Genet. 1979 Feb 1;169(3):289-97 PMID: 372751
-
Mutations affecting the formation of acetohydroxy acid synthase II in Escherichia coli K-12.Mol Gen Genet. 1979 Feb 1;169(3):299-314 PMID: 372752
-
Mapping of ilvO loci of Escherichia coli K-12 with bacteriophage lambda dilv.J Bacteriol. 1979 Mar;137(3):1234-42 PMID: 374344
-
Growth inhibition as a consequence of antagonism between related amino acids: effect of valine in Escherichia coli K-12.Microbiol Rev. 1979 Mar;43(1):42-58 PMID: 379577
-
Positive control of ilvC expression in Escherichia coli K-12; identification and mapping of regulatory gene ilvY.J Bacteriol. 1979 Sep;139(3):1014-20 PMID: 113381
-
Transcription termination in the Escherichia coli ribosomal RNA operon rrnC.J Biol Chem. 1979 Dec 25;254(24):12725-31 PMID: 115888
-
Nucleotide sequence of ilvGEDA operon attenuator region of Escherichia coli.Proc Natl Acad Sci U S A. 1980 Apr;77(4):1823-7 PMID: 6990415
-
Multivalent translational control of transcription termination at attenuator of ilvGEDA operon of Escherichia coli K-12.Proc Natl Acad Sci U S A. 1980 Apr;77(4):1862-6 PMID: 6154938
-
Linkage map of Escherichia coli K-12, edition 6.Microbiol Rev. 1980 Mar;44(1):1-56 PMID: 6997720
-
Molecular basis of valine resistance in Escherichia coli K-12.Proc Natl Acad Sci U S A. 1981 Feb;78(2):922-5 PMID: 7015336
-
Genetical and structural analysis of a group of lambda ilv and lambda rho transducing phages.Mol Gen Genet. 1981;182(3):462-70 PMID: 6272063
-
DNA sequence fine-structure analysis of ilvG (IlvG+) mutations of Escherichia coli K-12.J Bacteriol. 1982 Jan;149(1):294-8 PMID: 7033211
-
Physical and genetic localization of ilv regulatory sites in lambda ilv bacteriophages.J Bacteriol. 1982 Mar;149(3):1071-81 PMID: 6277848
-
Identification of the protein products of the rrnC, ilv, rho region of the Escherichia coli K-12 chromosome.Mol Gen Genet. 1981;183(3):428-36 PMID: 6460909
-
Absence of significant membrane localization of the proteins coded by the ilvGEDAC genes of Escherichia coli K-12.J Bacteriol. 1982 Jul;151(1):119-26 PMID: 6211429
-
Cloning of the ilvA538 gene coding for feedback-hypersensitive threonine deaminase from Escherichia coli K-12.J Bacteriol. 1982 Jul;151(1):274-80 PMID: 7045077
Contact
No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong
Qilu Normal University · Genelibs Bioinformatics Lab
750 Shunhua Rd, Jinan
2F, Bldg F, University Science Park
Tel: 0531-88819269
WeChat Official Account
Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.
Business Email
E-mail: product@genelibs.com
Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2
