Home LiteratureArticle Details
PMID: 6207534 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Antibodies to two defined regions of the transforming protein pp60src interact specifically with the epidermal growth factor receptor kinase system.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 81 ·No. 19 ·1984-10-00 ·Pages 5911-5

Lax I, Bar-Eli M, Yarden Y, Libermann TA, Schlessinger J

Abstract

Antibodies generated against two synthetic peptides corresponding to two defined regions on the transforming protein of Rous sarcoma virus, pp60src, interact specifically with the epidermal growth factor (EGF)-receptor kinase. An antibody directed against a synthetic peptide corresponding to the major phosphorylation site of pp60src interacts specifically with EGF receptor and immunoprecipitates a functional EGF-receptor kinase. The second antibody, which binds close to a region on the src molecule that is required for its kinase activity, also binds to EGF-receptor kinase and prevents the autophosphorylation of the receptor molecules. Neither antibody binds to intact cells, but they do recognize various forms of the solubilized receptor. It is concluded that at least two cytoplasmic domains of the EGF receptor are antigenically and presumably also structurally related to specific domains on pp60src.

MeSH Terms
Amino Acid Sequence Antibodies Antigen-Antibody Complex Carcinoma, Squamous Cell Cell Line Cell Membrane/metabolism Enzyme-Linked Immunosorbent Assay Epidermal Growth Factor/metabolism Epitopes/analysis ErbB Receptors Humans Oncogene Protein pp60(v-src) Phosphorylation Protein Kinases/immunology Receptors, Cell Surface/metabolism Viral Proteins/immunology
Chemicals
Antibodies Antigen-Antibody Complex Epitopes Receptors, Cell Surface Viral Proteins Epidermal Growth Factor Protein Kinases ErbB Receptors Oncogene Protein pp60(v-src)
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Lax I
Bar-Eli M
Yarden Y
Libermann T A
Schlessinger J
References (29)
29 references, click to expand
  1. Monoclonal antibody reactive with the human epidermal-growth-factor receptor recognizes the blood-group-A antigen.
    Biosci Rep. 1983 Nov;3(11):1045-52 PMID: 6198005
  2. Phosphorylation of tyrosine-416 is not required for the transforming properties and kinase activity of pp60v-src.
    Cell. 1983 Mar;32(3):891-901 PMID: 6403250
  3. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
    Nature. 1970 Aug 15;227(5259):680-5 PMID: 5432063
  4. Purified EGF receptor-kinase interacts specifically with antibodies to Rous sarcoma virus transforming protein.
    Nature. 1981 Apr 9;290(5806):516-9 PMID: 6261144
  5. Epidermal growth factor and a new derivative. Rapid isolation procedures and biological and chemical characterization.
    J Biol Chem. 1972 Dec 10;247(23):7609-11 PMID: 4636326
  6. Biosynthesis of the epidermal growth factor receptor in A431 cells.
    EMBO J. 1984 Mar;3(3):531-7 PMID: 6325174
  7. Avian sarcoma virus-transforming protein, pp60src shows protein kinase activity specific for tyrosine.
    Nature. 1980 May 15;285(5761):167-9 PMID: 6246443
  8. The purified product of the transforming gene of avian sarcoma virus phosphorylates tyrosine.
    J Biol Chem. 1980 Dec 25;255(24):11973-80 PMID: 6254988
  9. Appearance of functional EGF receptor kinase during rodent embryogenesis.
    EMBO J. 1983;2(11):1937-41 PMID: 6196193
  10. Stimulation of tyrosine-specific phosphorylation by platelet-derived growth factor.
    Nature. 1982 Feb 4;295(5848):419-20 PMID: 6173766
  11. Anti-pp60src antibodies are substrates for EGF-stimulated protein kinase.
    Nature. 1981 Apr 9;290(5806):519-21 PMID: 6261145
  12. Epidermal growth factor-receptor-protein kinase interactions. Co-purification of receptor and epidermal growth factor-enhanced phosphorylation activity.
    J Biol Chem. 1980 May 25;255(10):4834-42 PMID: 6246084
  13. Preparation of iodine-131 labelled human growth hormone of high specific activity.
    Nature. 1962 May 5;194:495-6 PMID: 14450081
  14. Regulation of cell proliferation by epidermal growth factor.
    CRC Crit Rev Biochem. 1983;14(2):93-111 PMID: 6301752
  15. Expression of epidermal growth factor receptors in human brain tumors.
    Cancer Res. 1984 Feb;44(2):753-60 PMID: 6318976
  16. Immunochemical studies on big gastrin using NH2-terminal specific antisera.
    Regul Pept. 1980 Dec;1(3):169-86 PMID: 6165051
  17. Antibodies to a defined region of pp60src neutralize the tyrosine-specific kinase activity.
    J Biol Chem. 1983 Sep 25;258(18):11219-28 PMID: 6411728
  18. Biological role of epidermal growth factor-receptor clustering. Investigation with monoclonal anti-receptor antibodies.
    J Biol Chem. 1983 Jan 25;258(2):846-53 PMID: 6296087
  19. Epidermal growth factor stimulates phosphorylation in membrane preparations in vitro.
    Nature. 1978 Nov 23;276(5686):409-10 PMID: 309559
  20. Stimulation of tyrosine-specific phosphorylation in vitro by insulin-like growth factor I.
    Nature. 1983 Sep 29-Oct 5;305(5933):438-40 PMID: 6312321
  21. Insulin stimulates tyrosine phosphorylation of the insulin receptor in a cell-free system.
    Nature. 1982 Aug 12;298(5875):667-9 PMID: 6178977
  22. A nonmitogenic analogue of epidermal growth factor induces early responses mediated by epidermal growth factor.
    J Cell Biol. 1982 Mar;92(3):687-93 PMID: 6282891
  23. Cellular oncogenes and retroviruses.
    Annu Rev Biochem. 1983;52:301-54 PMID: 6351725
  24. "Western blotting": electrophoretic transfer of proteins from sodium dodecyl sulfate--polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein A.
    Anal Biochem. 1981 Apr;112(2):195-203 PMID: 6266278
  25. Epidermal growth factor.
    Annu Rev Biochem. 1979;48:193-216 PMID: 382984
  26. A non-mitogenic analogue of epidermal growth factor enhances the phosphorylation of endogenous membrane proteins.
    Biochem Biophys Res Commun. 1981 Jul 30;101(2):517-23 PMID: 6272761
  27. A native 170,000 epidermal growth factor receptor-kinase complex from shed plasma membrane vesicles.
    J Biol Chem. 1982 Feb 10;257(3):1523-31 PMID: 6276390
  28. Transforming gene product of Rous sarcoma virus phosphorylates tyrosine.
    Proc Natl Acad Sci U S A. 1980 Mar;77(3):1311-5 PMID: 6246487
  29. Close similarity of epidermal growth factor receptor and v-erb-B oncogene protein sequences.
    Nature. 1984 Feb 9-15;307(5951):521-7 PMID: 6320011
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.81.19.5911
Published
1984-10-00
Pages
5911-5
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC391828
Subset
IM
Grants
NCI NIH HHS · CA-25820 · United States
External Links
PubMed source DOI: 10.1073/pnas.81.19.5911 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2