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PMID: 6199794 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Isolation of cDNA clones for human complement component C2.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 81 ·No. 4 ·1984-02-00 ·Pages 1212-5

Bentley DR, Porter RR

Abstract

Two cDNA clones for complement component C2 have been isolated from a high-complexity human liver cDNA library by using a mixture of 64 synthetic oligonucleotides as a probe. The 400-base-pair insert of pC201 codes for a region containing the active site serine residue and the secondary substrate binding pocket of the serine protease. This part of C2 is 34% homologous to the corresponding region of the related serine protease factor B and additional similarity is evident from a number of conservative amino acid replacements in this region. The insert of pC201 was used as a specific probe in RNA transfer analysis to determine the size of the C2 mRNA as approximately equal to 2.9 kilobases. Southern blot analysis of genomic DNA of unrelated individuals identified a single C2 locus and showed no cross-hybridization with the factor B locus.

MeSH Terms
Amino Acid Sequence Base Sequence Cloning, Molecular Complement C2/genetics DNA/isolation & purification DNA Restriction Enzymes Humans Liver/metabolism Nucleic Acid Hybridization Poly A/genetics RNA/genetics RNA, Messenger
Chemicals
Complement C2 RNA, Messenger Poly A RNA DNA DNA Restriction Enzymes
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Bentley D R
Porter R R
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30 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.81.4.1212
Published
1984-02-00
Pages
1212-5
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC344796
Subset
IM
Databases
GENBANK
K01236, X04481
External Links
PubMed source DOI: 10.1073/pnas.81.4.1212 PMC Full Text View journal details
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