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PMID: 6198633 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

DNA binding and antigenic specifications of DNA gyrase.

Nucleic acids research ·Vol. 12 ·No. 2 ·1984-01-25 ·Pages 901-14

Lother H, Lurz R, Orr E

Abstract

Complexes of DNA gyrase and minichromosomal DNA containing the origin of replication of Escherichia coli (oriC) can be formed without metabolic energy and visualised by electron microscopy. The A subunit, part of the A2B2-DNA gyrase complex is the binding protein. Various binding sites are scattered around the minichromosomal DNA including oriC. The minimal origin contains the only prominent and reproducible binding site. Binding to this site is suppressed by oxolinic acid and the ATP analogue beta-y-imido ATP. If gyrase isolated from the gram-positive bacterium Bacillus subtilis is used no binding to oriC is seen. This observation is consistent with antigenic differences between the A subunits of the two microorganisms. The binding to oriC might reflect a requirement for DNA gyrase during the initiation of DNA replication.

MeSH Terms
Antigen-Antibody Complex Bacillus subtilis/enzymology Base Sequence DNA DNA Topoisomerases, Type II/genetics,metabolism Epitopes/analysis Escherichia coli/enzymology Macromolecular Substances Microscopy, Electron Nucleic Acid Hybridization Protein Binding Substrate Specificity
Chemicals
Antigen-Antibody Complex Epitopes Macromolecular Substances DNA DNA Topoisomerases, Type II
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Lother H
Lurz R
Orr E
References (33)
33 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/12.2.901
Published
1984-01-25
Pages
901-14
Language
English
Region
England
NLM ID
0411011
PMCID
PMC318544
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/12.2.901 PMC Full Text View journal details
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