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PMID: 6188110 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Structural analysis of gene loci for rat U1 small nuclear RNA.

Nucleic acids research ·Vol. 11 ·No. 6 ·1983-03-25 ·Pages 1791-801

Watanabe-Nagasu N, Itoh Y, Tani T, Okano K, Koga N, Okada N, Ohshima Y

Abstract

Four phage clones which hybridize with U1 small nuclear RNA were obtained from a rat gene library. Two clones contain a presumed pseudogene. A third clone includes two gene candidates that are co-linear with the rat U1-RNA, 3.6kb apart and in the opposite orientation. The two genes are surrounded by identical sequences of 491bp upstream and 178bp downstream. The upstream sequences do not contain a TATA box, but share many block homologies with those for the human U1-RNA gene(1-3). A 101bp "identifier (ID) sequence", which was reported to be specifically expressed in rat brain (4), is inserted immediately after the shared sequence downstream of one of the genes. In the fourth clone, there are two putative pseudogenes, which have one or three nucleotide changes, 3kb apart and in the same orientation. Southern blot analysis of total rat DNA reveals about 50 U1-RNA genes/pseudogenes in the genome.

MeSH Terms
Animals Cloning, Molecular Coliphages/genetics DNA Restriction Enzymes Genes Liver/metabolism Nucleic Acid Hybridization RNA/genetics RNA, Small Nuclear Rats Rats, Inbred Strains
Chemicals
RNA, Small Nuclear RNA DNA Restriction Enzymes
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Watanabe-Nagasu N
Itoh Y
Tani T
Okano K
Koga N
Okada N
Ohshima Y
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37 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/11.6.1791
Published
1983-03-25
Pages
1791-801
Language
English
Region
England
NLM ID
0411011
PMCID
PMC325836
Subset
IM
Databases
GENBANK
J00800, K02430, K02431, K02432, K02433
External Links
PubMed source DOI: 10.1093/nar/11.6.1791 PMC Full Text View journal details
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