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PMID: 6181509 Published · ppublish English Journal Article

Phospholipids stimulate phosphorylation of vinculin by the tyrosine-specific protein kinase of Rous sarcoma virus.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 79 ·No. 15 ·1982-08-00 ·Pages 4628-31

Ito S, Richert N, Pastan I

Abstract

The phosphorylation of vinculin by a highly purified tyrosine-specific protein kinase was enhanced more than 10-fold by anionic phospholipids: the phosphorylation of casein, actin, and alpha-actinin was inhibited. The effect of phospholipid was dependent on the divalent cation used. Stimulation was observed by phosphatidylinositol or phosphatidylglycerol in the presence of either 0.5 mM Mn2+ of 5 mM Mg2+; with either phospholipid, more enzyme activity was observed with Mn2+. Maximal stimulation by phosphatidylinositol was observed at about 400 micrograms/ml. In contrast, marked stimulation by phosphatidylserine was observed only with Mn2+ and marked stimulation by phosphatidic acid was observed only with Mg2+. These results raise the possibility that phospholipids modulate vinculin phosphorylation in Rous sarcoma virus-transformed cells.

MeSH Terms
Avian Sarcoma Viruses/enzymology Cell Transformation, Viral Enzyme Activation Magnesium/pharmacology Manganese/pharmacology Muscle Proteins/metabolism Phosphatidylinositols/pharmacology Phospholipids/pharmacology Phosphorylation Phosphotyrosine Protein Kinases/metabolism Substrate Specificity Tyrosine/analogs & derivatives,metabolism Vinculin
Chemicals
Muscle Proteins Phosphatidylinositols Phospholipids Vinculin Phosphotyrosine Tyrosine Manganese Protein Kinases Magnesium
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Ito S
Richert N
Pastan I
References (29)
29 references, click to expand
  1. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
    Nature. 1970 Aug 15;227(5259):680-5 PMID: 5432063
  2. The regulation of rabbit skeletal muscle contraction. I. Biochemical studies of the interaction of the tropomyosin-troponin complex with actin and the proteolytic fragments of myosin.
    J Biol Chem. 1971 Aug 10;246(15):4866-71 PMID: 4254541
  3. Purification and characterization of an inhibitor protein of cyclic AMP-dependent protein kinases.
    Methods Enzymol. 1974;38:350-8 PMID: 4375767
  4. Inositol phospholipids and cell surface receptor function.
    Biochim Biophys Acta. 1975 Mar 25;415(1):81-47 PMID: 164246
  5. Identification of a transformation-specific antigen induced by an avian sarcoma virus.
    Nature. 1977 Sep 22;269(5626):346-8 PMID: 198667
  6. Protein kinase activity associated with the avian sarcoma virus src gene product.
    Proc Natl Acad Sci U S A. 1978 Apr;75(4):2021-4 PMID: 205879
  7. Evidence that the transforming gene of avian sarcoma virus encodes a protein kinase associated with a phosphoprotein.
    Cell. 1978 Oct;15(2):561-72 PMID: 214242
  8. The participation of alpha-actinin in the capping of cell membrane components.
    Cell. 1979 Jan;16(1):213-22 PMID: 369707
  9. Calcium-dependent activation of a multifunctional protein kinase by membrane phospholipids.
    J Biol Chem. 1979 May 25;254(10):3692-5 PMID: 438153
  10. Characterization of the protein kinase activity of avian sarcoma virus src gene product.
    Proc Natl Acad Sci U S A. 1979 Oct;76(10):5028-32 PMID: 228274
  11. Localization of the ASV src gene product to the plasma membrane of transformed cells by electron microscopic immunocytochemistry.
    Cell. 1979 Sep;18(1):125-34 PMID: 228858
  12. A 130K protein from chicken gizzard: its localization at the termini of microfilament bundles in cultured chicken cells.
    Cell. 1979 Sep;18(1):193-205 PMID: 574428
  13. Characterization of an immune complex kinase in immunoprecipitates of avian sarcoma virus-transformed fibroblasts.
    J Virol. 1979 Sep;31(3):696-706 PMID: 41953
  14. A rapid purification of alpha-actinin, filamin, and a 130,000-dalton protein from smooth muscle.
    J Biol Chem. 1980 Feb 10;255(3):1194-9 PMID: 7356657
  15. Evidence that the avian sarcoma virus transforming gene product is a cyclic AMP-independent protein kinase.
    Proc Natl Acad Sci U S A. 1979 Dec;76(12):6260-4 PMID: 230504
  16. Evidence that the src gene product of Rous sarcoma virus is membrane associated.
    Virology. 1980 Feb;101(1):25-40 PMID: 6243822
  17. Microinjection and localization of a 130K protein in living fibroblasts: a relationship to actin and fibronectin.
    Cell. 1980 Mar;19(3):587-95 PMID: 6988083
  18. Avian sarcoma virus-transforming protein, pp60src shows protein kinase activity specific for tyrosine.
    Nature. 1980 May 15;285(5761):167-9 PMID: 6246443
  19. Transforming gene product of Rous sarcoma virus phosphorylates tyrosine.
    Proc Natl Acad Sci U S A. 1980 Mar;77(3):1311-5 PMID: 6246487
  20. Evidence that the phosphorylation of tyrosine is essential for cellular transformation by Rous sarcoma virus.
    Cell. 1980 Jul;20(3):807-16 PMID: 6251974
  21. The protein encoded by the transforming gene of avian sarcoma virus (pp60src) and a homologous protein in normal cells (pp60proto-src) are associated with the plasma membrane.
    Proc Natl Acad Sci U S A. 1980 Jul;77(7):3783-7 PMID: 6253989
  22. The purified product of the transforming gene of avian sarcoma virus phosphorylates tyrosine.
    J Biol Chem. 1980 Dec 25;255(24):11973-80 PMID: 6254988
  23. Altered distributions of the cytoskeletal proteins vinculin and alpha-actinin in cultured fibroblasts transformed by Rous sarcoma virus.
    Proc Natl Acad Sci U S A. 1980 Nov;77(11):6687-91 PMID: 6256755
  24. Structural and functional domains of the Rous sarcoma virus transforming protein (pp60src).
    Proc Natl Acad Sci U S A. 1981 Mar;78(3):1624-8 PMID: 6262821
  25. Vinculin: a cytoskeletal target of the transforming protein of Rous sarcoma virus.
    Cell. 1981 Apr;24(1):165-74 PMID: 6263485
  26. Interaction of alpha-actinin and vinculin with actin: opposite effects on filament network formation.
    Proc Natl Acad Sci U S A. 1981 May;78(5):3005-9 PMID: 6789327
  27. Biochemical characterization of pp60src-associated protein kinase from avian sarcoma virus Schmidt-Ruppin strain.
    J Biol Chem. 1981 Aug 25;256(16):8786-94 PMID: 6267051
  28. High-affinity interaction of vinculin with actin filaments in vitro.
    Cell. 1982 Jan;28(1):83-90 PMID: 6802502
  29. Changes in protein phosphorylation in Rous sarcoma virus-transformed chicken embryo cells.
    Mol Cell Biol. 1981 Feb;1(2):165-78 PMID: 6100962
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.79.15.4628
Published
1982-08-00
Pages
4628-31
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC346728
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.79.15.4628 PMC Full Text View journal details
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