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PMID: 6181051 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Regulatory region of the Klebsiella aerogenes tryptophan operon.

Journal of bacteriology ·Vol. 152 ·No. 1 ·1982-10-00 ·Pages 49-56

Blumenberg M, Yanofsky C

Abstract

The trp operon of Klebsiella aerogenes was cloned, and its regulatory region was sequenced. Comparison with previously reported trp regulatory sequences of other enteric bacteria indicates that the K. aerogenes trp promoter-operator region is most similar to the corresponding region of Salmonella typhimurium. The trp leader regions of K. aerogenes and other enteric bacteria are organized similarly, but there are significant differences in the stabilities of the predicted secondary structures in their leader transcripts. These differences should make the K. aerogenes attenuator a weaker transcription termination site than any of the other attenuator regions studied; this was confirmed in in vitro transcription experiments. The sequence of the leader transcript and the precise site of in vitro termination were determined.

MeSH Terms
Base Sequence Cloning, Molecular DNA, Bacterial Genes, Bacterial Genes, Regulator Klebsiella pneumoniae/genetics Operon RNA, Bacterial/genetics Transcription, Genetic Tryptophan/biosynthesis
Chemicals
DNA, Bacterial RNA, Bacterial Tryptophan
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Blumenberg M
Yanofsky C
References (19)
19 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.152.1.49-56.1982
Published
1982-10-00
Pages
49-56
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC221373
Subset
IM
Grants
NIGMS NIH HHS · GM09738 · United States
Databases
GENBANK
J01739
External Links
PubMed source DOI: 10.1128/jb.152.1.49-56.1982 PMC Full Text View journal details
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