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PMID: 6180180 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Polypurine tract adjacent to the U3 region of the Rous sarcoma virus genome provides a cis-acting function.

Journal of virology ·Vol. 43 ·No. 2 ·1982-08-00 ·Pages 482-8

Sorge J, Hughes SH

Abstract

The v-src coding region was deleted from cloned Rous sarcoma virus DNA, and the deleted clones were tested for infectivity by transfection. All of the coding region, as well as most of the sequences lying between v-src and the unique 3' region (U3), could be deleted without affecting viability. However, at least 9 and at most 29 of the nucleotides in the purine-rich tract adjacent to U3 were necessary for growth, even in the presence of a helper virus. It is concluded that these nucleotides (the polypurine tract) provide a cis-acting function necessary for retrovirus replication.

MeSH Terms
Animals Avian Sarcoma Viruses/genetics,growth & development Base Sequence Cell Line Chick Embryo Cloning, Molecular DNA, Viral/genetics Genes, Viral Mutation Purines RNA, Viral/genetics RNA-Directed DNA Polymerase/metabolism Transfection
Chemicals
DNA, Viral Purines RNA, Viral RNA-Directed DNA Polymerase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Sorge J
Hughes S H
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22 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.43.2.482-488.1982
Published
1982-08-00
Pages
482-8
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC256151
Subset
IM
Grants
NCI NIH HHS · 1 F32 CA06875 · United States
NCI NIH HHS · CA 29569-02 · United States
External Links
PubMed source DOI: 10.1128/JVI.43.2.482-488.1982 PMC Full Text View journal details
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