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PMID: 6179044 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Changes induced in rat liver polysomal polyadenylated RNAs by depletion of circulating glucocorticoids.

Nucleic acids research ·Vol. 10 ·No. 11 ·1982-06-11 ·Pages 3541-60

Bajwa W, Birnie GD, Knowler JT

Abstract

The effects of adrenalectomy on the complexity and the relative abundances of rat liver polyadenylated mRNAs have been investigated. The qualitative and quantitative changes induced by adrenalectomy have been measured by hybridisation of polysomal polyadenylated RNAs from the livers of normal and adrenalectomised rats with total cDNAs, fractionated cDNAs, cDNA representing RNAs specific to normal liver, total unique-sequence DNA and unique-sequence DNA complementary to normal liver polysomal RNA. These analyses indicated that, by 14 days after adrenalectomy, the equivalent of about 7000 sequences of average length 2000 nucleotides can no longer be detected in liver polysomes. Many other sequences are decreased in abundance as compared to normal liver, but some abundant sequences become more abundant. Administration of a glucocorticoid hormone (dexamethasone) very rapidly reverses these changes.

MeSH Terms
Adrenalectomy Animals Base Sequence DNA Glucocorticoids/blood Kinetics Liver/metabolism Male Nucleic Acid Hybridization Poly A/metabolism Polyribosomes/metabolism RNA/metabolism RNA, Messenger/metabolism Rats Rats, Inbred Strains
Chemicals
Glucocorticoids RNA, Messenger Poly A RNA DNA
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Bajwa W
Birnie G D
Knowler J T
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/10.11.3541
Published
1982-06-11
Pages
3541-60
Language
English
Region
England
NLM ID
0411011
PMCID
PMC320730
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/10.11.3541 PMC Full Text View journal details
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