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PMID: 170919 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Glucocorticoids and the regulation of phosphoenolpyruvate carboxykinase (guanosine triphosphate) in the rat.

The Biochemical journal ·Vol. 150 ·No. 2 ·1975-08-00 ·Pages 195-203

Gunn JM, Hanson RW, Meyuhas O, Reshef L, Ballard FJ

Abstract

The effect glucocorticoids on the synthesis and degradation of phosphoenolpyruvate carboxykinase (GTP)(EC4.1.1.32) in rat liver and kidney in vivo was studied immunochemically. The glucocorticoid analogue triamcinolone (9alpha-fluoro-11beta, 21-dihydroxy-16alpha,17alpha-isopropylidenedioxypregna-1,4-diene-3,20-dione) increased the synthesis rate of the kidney enzyme in starved animals. Both triamcinolone and cortisol decreased the synthesis rate of hepatic phosphoenolpyruvate carboxykinase (GTP) in fed and starved rats, but were without effect on the degradation rate of the enzyme. This effect of triamcinolone in liver was reversed by injection of dibutyryl cyclic AMP. However, in diabetic animals glucocorticoids increased the synthesis rate of hepatic phosphoenolpyruvate carboxykinase (GTP). Triamcinolone administration to starved rats in vivo is shown to cause an increase in the portal blood concentrations of insulin and glucose. Since the physiological de-inducer of liver phosphoenolpyruvate carboxykinase (GTP) is insulin, this is the probable cause of the decrease in the synthesis rate of the hepatic enzyme noted when glucocorticoids are administered to non-diabetic animals.

MeSH Terms
Animals Blood Glucose/metabolism Bucladesine/pharmacology Cytosol/enzymology Dose-Response Relationship, Drug Enzyme Induction/drug effects Guanosine Triphosphate Hydrocortisone/pharmacology Insulin/blood Kidney/drug effects,enzymology Liver/drug effects,enzymology Male Phosphoenolpyruvate Carboxykinase (GTP)/immunology,metabolism Rats Triamcinolone Acetonide/pharmacology
Chemicals
Blood Glucose Insulin Bucladesine Guanosine Triphosphate Phosphoenolpyruvate Carboxykinase (GTP) Triamcinolone Acetonide Hydrocortisone
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Gunn J M
Hanson R W
Meyuhas O
Reshef L
Ballard F J
References (43)
43 references, click to expand
  1. Deinduction of phosphoenolpyruvate carboxykinase (guanosine triphosphate) synthesis in Reuber H-35 cells.
    J Biol Chem. 1975 May 10;250(9):3322-9 PMID: 164466
  2. Insulin secretion in vitro by pancreatic tissue from normal, adrenalectomized, and cortisol-treated rats.
    Proc Soc Exp Biol Med. 1967 Mar;124(3):924-8 PMID: 6024801
  3. Interaction between hormones and cyclic AMP in regulating specific hepatic enzyme synthesis.
    Fed Proc. 1974 Apr;33(4):1105-11 PMID: 4150434
  4. Interaction of hydrocortisone and dibutyryl cyclic AMP in the induction of tyrosine amino-transferase and phosphoenolpyruvate carboxy-kinase in rat liver.
    FEBS Lett. 1974 Sep 15;46(1):158-61 PMID: 4153975
  5. Purification of phosphoenolpyruvate carboxykinase from the cytosol fraction of rat liver and the immunochemical demonstration of differences between this enzyme and the mitochondrial phosphoenolpyruvate carboxykinase.
    J Biol Chem. 1969 Oct 25;244(20):5625-30 PMID: 4186849
  6. Renal cortical and hepatic phosphoenolpyruvate carboxylase in the diabetic rat: effect of acid-base status.
    Metabolism. 1974 Nov;23(11):1073-9 PMID: 4214045
  7. Studies on the role of adenosine 3',5'-monophosphate in the hepatic actions of glucagon and catecholamines.
    J Biol Chem. 1971 Oct 25;246(20):6166-77 PMID: 4331382
  8. Relationship of some hepatic actions of insulin to the intracellular level of cyclic adenylate.
    Diabetes. 1972;21(2 Suppl):439-46 PMID: 4340999
  9. The interaction of catecholamines and adrenal corticosteroids in the induction of phosphopyruvate carboxylase in rat liver and adipose tissue.
    Biochem J. 1972 May;127(5):809-18 PMID: 4342497
  10. The relationship between insulin and apparent glucocorticoid-promoted activation of hepatic glycogen synthetase.
    Biochim Biophys Acta. 1972 Sep 15;279(2):245-59 PMID: 4343213
  11. Interaction of adrenal steroids and glucagon on gluconeogenesis in perfused rat liver.
    Biochem Biophys Res Commun. 1967 Oct 11;29(1):113-9 PMID: 6055176
  12. Hormonal control of amino acid metabolism.
    Fed Proc. 1955 Sep;14(3):696-705; discussion, 705-6 PMID: 13262130
  13. Regulation of carbohydrate metabolism in isolated tissues.
    Vitam Horm. 1956;14:139-85 PMID: 13409776
  14. Plasma half-life, tissue distribution, and excretion of triamcinolone-H3.
    J Pharmacol Exp Ther. 1961 Mar;131:287-93 PMID: 13700366
  15. An automated determination of glucose utilizing a glucose oxidase-peroxidase system.
    J Lab Clin Med. 1961 Jun;57:970-80 PMID: 13714284
  16. METABOLIC AND HORMONAL CONTROL OF PHOSPHOENOLPYRUVATE CARBOXYKINASE AND MALIC ENZYME IN RAT LIVER.
    J Biol Chem. 1963 Oct;238:3188-92 PMID: 14085360
  17. MODE OF ACTION OF GLUCOCORTICOIDS. I. STIMULATION OF GLUCONEOGENESIS INDEPENDENT OF SYNTHESIS DE NOVO OF ENZYMES.
    J Biol Chem. 1964 Oct;239:3396-400 PMID: 14245393
  18. Synthesis and degradation of phosphoenolpyruvate carboxylase in rat liver and adipose tissue. Changes during a starvation-re-feeding cycle.
    Biochem J. 1973 Jun;134(2):445-53 PMID: 16742804
  19. The effect of steroids and ammonium chloride acidosis on phosphoenolpyruvate carboxykinase in rat kidney cortex. II. The kinetics of enzyme induction.
    J Clin Invest. 1972 Sep;51(9):2284-91 PMID: 4344728
  20. Regulation of phospho-enol-pyruvate carboxykinase during starvation and glucose repression.
    Nat New Biol. 1972 Oct 4;239(92):131-3 PMID: 4349670
  21. Dynamics of insulin release by perfused rat pancreases: effects of hypophysectomy, growth hormone, adrenocorticotropic hormone, and hydrocortisone.
    Endocrinology. 1973 Sep;93(3):602-9 PMID: 4352804
  22. Effects of adrenalectomy and glucocorticoid replacement on gluconeogenesis in perfused livers from diabetic rats.
    Biochim Biophys Acta. 1973 Nov 2;329(1):41-57 PMID: 4361567
  23. The regulation of phosphoenolpyruvate carboxykinase in fetal rat liver.
    Enzyme. 1973;15(1):97-110 PMID: 4361643
  24. Rapid loss of translatable messenger RNA of phosphoenolpyruvate carboxykinase during glucose repression in liver.
    Proc Natl Acad Sci U S A. 1974 Apr;71(4):1304-8 PMID: 4364533
  25. Stimulation by 6-N,2'-O-dibutyryladenosine 3' :5'-cyclic monophosphate and glucocorticoids of phosphoenolpyruvate carboxykinase in the isolated perfused rat liver.
    Biochem J. 1974 Sep;142(3):691-8 PMID: 4377216
  26. Induction of hepatic enzyme synthesis in vivo by adenosine 3', 5'-monophosphate.
    J Biol Chem. 1969 Nov 10;244(21):6008-13 PMID: 4390807
  27. Regulation of phosphoenolpyruvate carboxykinase and tyrosine transaminase in hepatoma cell cultures. I. Effects of glucocorticoids, N 6 ,O 2' -dibutyryl cyclic adenosine 3',5'-monophosphate and insulin in Reuber H35 cells.
    J Biol Chem. 1971 Dec 10;246(23):7201-6 PMID: 4399619
  28. Induction of phosphoenolpyruvate carboxykinase by N 6 , O 2 '-dibutyryl cyclic AMP in rat liver.
    Biochim Biophys Acta. 1972 Mar 30;264(1):177-85 PMID: 4401804
  29. Evidence for translational regulation of specific enzyme synthesis by N 6 , O 2' -dibutyryl cyclic AMP in hepatoma cell cultures.
    Biochem Biophys Res Commun. 1972 Jul 11;48(1):205-11 PMID: 4402866
  30. The effect of steroids and ammonium chloride acidosis on phosphoenolpyruvate carboxykinase in rat kidney cortex. I. Differentiation of the inductive process and characterization of enzyme activities.
    J Clin Invest. 1972 Sep;51(9):2277-83 PMID: 4639014
  31. Metabolic effects of exogenous glucocorticoids in fasted man.
    J Clin Invest. 1973 Oct;52(10):2596-605 PMID: 4729053
  32. Phosphopyruvate carboxylase induction by L-tryptophan. Effects on synthesis and degradation of the enzyme.
    Biochem J. 1973 Oct;136(2):259-64 PMID: 4774395
  33. Alteration by fasting of the effects of methylprednisolone on carbohydrate metabolism in the normal dog.
    Metabolism. 1974 Apr;23(4):369-74 PMID: 4817366
  34. Dependence on insulin of the apparent hydrocortisone activation of hepatic glycogen synthetase.
    Proc Natl Acad Sci U S A. 1967 Oct;58(4):1515-9 PMID: 5237884
  35. The role of the adrenals in the regulation of phosphoenolpyruvate carboxykinase of rat adipose tissue.
    J Biol Chem. 1969 Oct 25;244(20):5577-81 PMID: 5348601
  36. A possible physiological role for glyceroneogenesis in rat adipose tissue.
    J Biol Chem. 1970 Nov 25;245(22):5979-84 PMID: 5484457
  37. Role of adenosine 3',5'-monophosphate in the effects of insulin and anti-insulin serum on liver metabolism.
    J Biol Chem. 1968 Mar 10;243(5):1031-8 PMID: 5640968
  38. Effect of adrenalectomy on glucose turnover, the Cori cycle, and gluconeogenesis from alanine.
    Biochim Biophys Acta. 1969 Feb 18;177(1):11-6 PMID: 5781183
  39. Critical variables in the radioimmunoassay of serum insulin using the double antibody technic.
    Diabetes. 1965 Dec;14(12):771-9 PMID: 5850216
  40. The enzymatic carboxylation of phosphoenolpyruvate. II. Purification and properties of liver mitochondrial phosphoenolpyruvate carboxykinase.
    J Biol Chem. 1966 May 25;241(10):2413-20 PMID: 5911620
  41. Studies on the mechanisms underlying adaptive changes in rat liver phosphoenolpyruvate carboxykinase.
    Biochemistry. 1966 Feb;5(2):555-62 PMID: 5940940
  42. Hyperinsulinemia with diabetes induced by cortisone, and the influence of growth hormone in the Chinese hamster.
    Endocrinology. 1966 Oct;79(4):749-56 PMID: 5957189
  43. Effects of cyclic adenosine monophosphate, dexamethasone and insulin on phosphoenolpyruvate carboxykinase synthesis in Reuber H-35 hepatoma cells.
    Biochemistry. 1975 Jun 3;14(11):2350-7 PMID: 166654
Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj1500195
Published
1975-08-00
Pages
195-203
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1165726
Subset
IM
External Links
PubMed source DOI: 10.1042/bj1500195 PMC Full Text View journal details
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