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PMID: 6177004 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Assignment of the gene for beta 2-microglobulin (B2m) to mouse chromosome 2.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 79 ·No. 6 ·1982-03-00 ·Pages 1930-4

Cox DR, Sawicki JA, Yee D, Appella E, Epstein CJ

Abstract

We have assigned the gene (B2m) coding for murine beta 2-microglobulin (B2M) to mouse chromosome 2 by using a novel panel of Chinese hamster-mouse somatic cell hybrid clones. Because of 35 independent primary hybrids used in this study were derived from two types of feral mice, each with a different combination of Robertsonian translocation chromosomes, as well as from mice with a normal complement of acrocentric chromosomes, analysis of 16 selected mouse enzyme markers provided data on the segregation of all 20 mouse chromosomes in these hybrids. Mouse B2M was identified in cell hybrids by immunoprecipitation with a species-specific anti-mouse B2M antiserum followed by two-dimensional polyacrylamide gel electrophoresis of the immunoprecipitated polypeptides. Enzyme analysis of the segregant clones excluded all chromosomes for B2m assignment except mouse chromosome 2, and karyotype analysis of nine informative hybrid clones confirmed the assignment of B2m to this chromosome. These results demonstrate that, in the mouse, as in man, B2m is not linked to the major histocompatibility or immunoglobulin loci.

MeSH Terms
Animals Beta-Globulins/genetics Chromosome Mapping Cricetinae Cricetulus Genes Genetic Linkage Hybrid Cells Karyotyping Mice/genetics beta 2-Microglobulin/genetics
Chemicals
Beta-Globulins beta 2-Microglobulin
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Cox D R
Sawicki J A
Yee D
Appella E
Epstein C J
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30 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.79.6.1930
Published
1982-03-00
Pages
1930-4
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC346095
Subset
IM
Grants
NIGMS NIH HHS · GM-07085 · United States
NIGMS NIH HHS · GM-24309 · United States
External Links
PubMed source DOI: 10.1073/pnas.79.6.1930 PMC Full Text View journal details
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