Home LiteratureArticle Details
PMID: 6154769 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Lymphocyte membrane IgG and secreted IgG are structurally and allotypically distinct.

The Journal of experimental medicine ·Vol. 151 ·No. 5 ·1980-05-01 ·Pages 1260-74

Oi VT, Bryan VM, Herzenberg LA, Herzenberg LA

Abstract

We have demonstrated that there are structurally distinct membrane and secreted IgG2a immunoglobulin molecules. The membrane heavy chain is both larger and more acidic than the secreted molecule. This difference is not a result of different N-glycosidic-linked oligosaccharide chains. The membrane heavy chain also is antigenically different from its secreted homologue. This is based on the fact that secreted IgG2a molecules express an allotypic determinant absent on membrane molecules. We discussed the genetic control and gene organization of membrane and secreted immunoglobulin heavy chain sequences and suggest mechanisms controlling the expression of the simian virus 40 genome as models for alternate gene expression of membrane and secreted heavy chain polypeptide chains from the same DNA sequence. The possible biological significance of the membrane immunoglobulin acting as a recognition site for regulatory T cells also is discussed. The difference between membrane and secreted immunoglobulin is proposed as a possible explanation for the manner in which T cells interact with IgG on memory B cells in the presence of a large excess of IgG present in body fluids.

MeSH Terms
Animals Antibody Formation Epitopes Glycoproteins/immunology,metabolism Immunoglobulin Allotypes/analysis Immunoglobulin G/metabolism Isoelectric Point Lymphocytes/immunology Membrane Proteins/immunology Molecular Weight Receptors, Antigen, B-Cell/analysis
Chemicals
Epitopes Glycoproteins Immunoglobulin Allotypes Immunoglobulin G Membrane Proteins Receptors, Antigen, B-Cell
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Oi V T
Bryan V M
Herzenberg L A
Herzenberg L A
References (49)
49 references, click to expand
  1. The carrier effect in the secondary response to hapten-protein conjugates. I. Measurement of the effect with transferred cells and objections to the local environment hypothesis.
    Eur J Immunol. 1971 Jan;1(1):10-7 PMID: 14978856
  2. The requirement of more than one antigenic determinant for immunogenicity.
    J Exp Med. 1969 Jun 1;129(6):1131-43 PMID: 4181830
  3. Demonstration of heavy and light chain antigenic determinants on the cell-bound receptor for antigen. Similarities between membrane-attached and humoral antibodies produced by the same cell.
    J Exp Med. 1970 Dec 1;132(6):1233-49 PMID: 4101973
  4. Class, subclass, and allelic exclusion of membrane-bound Ig of human B lymphocytes.
    J Exp Med. 1972 Aug 1;136(2):409-14 PMID: 4557774
  5. Synthesis, assembly and secretion of mouse immunoglobulin.
    Transplant Rev. 1973;14:163-83 PMID: 4728820
  6. Synthesis, surface deposition and secretion of immunoglobulin M in bone marrow-derived lymphocytes before and after mitogenic stimulation.
    Transplant Rev. 1973;14:76-130 PMID: 4542309
  7. Regulation of antibody response in vitro. VI. Carrier-specific helper cells for IgG and IgE antibody response.
    J Immunol. 1973 Sep;111(3):720-32 PMID: 4126287
  8. Immunoglobulin (Ig) allotype markers on rabbit lymphocytes: separation of cells bearing different allotypes and demonstration of the binding of Ig to lymphoid cell membranes.
    J Immunol. 1973 Nov;111(5):1334-48 PMID: 4126772
  9. Membrane immunoglobulins of B lymphocytes: inability to detect certain characteristic IgM and IgD antigens.
    J Exp Med. 1974 Oct 1;140(4):895-903 PMID: 4139226
  10. Membrane immunoglobulins and antigen receptors on B and T lymphocytes.
    Adv Immunol. 1974;19(0):67-216 PMID: 4139885
  11. High resolution two-dimensional electrophoresis of proteins.
    J Biol Chem. 1975 May 25;250(10):4007-21 PMID: 236308
  12. Tunicamycin inhibition of polyisoprenyl N-acetylglucosaminyl pyrophosphate formation in calf-liver microsomes.
    Biochem Biophys Res Commun. 1975 Jul 8;65(1):248-57 PMID: 167767
  13. Cell surface immunoglobulin. XVI. Polypeptide chain structure of mouse IgM and IgD-like molecule.
    J Immunol. 1976 Feb;116(2):409-15 PMID: 814162
  14. Immunoglobulin-receptors revisited.
    Science. 1975 Sep 19;189(4207):964-9 PMID: 1083069
  15. Cell surface immunoglobulin. XVIII. Functional differences of B lymphocytes bearing different surface immunoglobulin isotypes.
    J Exp Med. 1976 May 1;143(5):1055-65 PMID: 1083417
  16. Mouse myeloma mutants blocked in the assembly, glycosylation and secretion of immunoglobulin.
    J Mol Biol. 1976 Apr 5;102(2):237-52 PMID: 818392
  17. T-cell regulation of antibody responses: demonstration of allotype-specific helper T cells and their specific removal by suppressor T cells.
    J Exp Med. 1976 Aug 1;144(2):330-44 PMID: 1085324
  18. Comparative aspects of glycoprotein structure.
    Annu Rev Biochem. 1976;45:217-37 PMID: 786150
  19. The role of polyprenol-linked sugars in glycoprotein synthesis.
    Annu Rev Biochem. 1976;45:95-112 PMID: 786163
  20. Demonstration that IgG memory is carried by IgG-bearing cells.
    Eur J Immunol. 1976 Jul;6(7):467-72 PMID: 1086788
  21. Cell membrane antigen isolation with the staphylococcal protein A-antibody adsorbent.
    J Immunol. 1976 Nov;117(5 Pt 1):1482-90 PMID: 187693
  22. Charge shift electrophoresis: simple method for distinguishing between amphiphilic and hydrophilic proteins in detergent solution.
    Proc Natl Acad Sci U S A. 1977 Feb;74(2):529-32 PMID: 265517
  23. Cell surface immunoglobulin. XX. Antibody responsiveness of subpopulations of B lymphocytes bearing different isotypes.
    J Exp Med. 1977 Jun 1;145(6):1421-35 PMID: 301171
  24. Monogamous T helper cell.
    Nature. 1977 Aug 18;268(5621):641-2 PMID: 302418
  25. Analysis of H-2 and Ia molecules by two-dimensional gel electrophoresis.
    J Exp Med. 1977 Nov 1;146(5):1261-79 PMID: 411873
  26. IgD and B cell differentiation.
    Immunol Rev. 1977;37:50-88 PMID: 73499
  27. High resolution two-dimensional electrophoresis of basic as well as acidic proteins.
    Cell. 1977 Dec;12(4):1133-41 PMID: 23215
  28. Proposal for a common oligosaccharide intermediate in the synthesis of membrane glycoproteins.
    Cell. 1977 Dec;12(4):893-900 PMID: 202393
  29. Processing of high mannose oligosaccharides to form complex type oligosaccharides on the newly synthesized polypeptides of the vesicular stomatitis virus G protein and the IgG heavy chain.
    J Biol Chem. 1978 Feb 10;253(3):716-22 PMID: 202596
  30. Organization and expression of early genes of simian virus 40.
    Proc Natl Acad Sci U S A. 1978 Jan;75(1):117-21 PMID: 203926
  31. Protein and cell membrane iodinations with a sparingly soluble chloroamide, 1,3,4,6-tetrachloro-3a,6a-diphrenylglycoluril.
    Biochem Biophys Res Commun. 1978 Feb 28;80(4):849-57 PMID: 637870
  32. Spliced early mRNAs of simian virus 40.
    Proc Natl Acad Sci U S A. 1978 Mar;75(3):1274-8 PMID: 206891
  33. Properties of monoclonal antibodies to mouse Ig allotypes, H-2, and Ia antigens.
    Curr Top Microbiol Immunol. 1978;81:115-20 PMID: 567555
  34. Effect of tunicamycin on IgM, IgA, and IgG secretion by mouse plasmacytoma cells.
    J Immunol. 1978 Sep;121(3):990-6 PMID: 690446
  35. Evidence for a C-terminal tyrosine residue in human and mouse B-lymphocyte membrane mu chains.
    Nature. 1978 Apr 6;272(5653):555-7 PMID: 99661
  36. Isolation of pure IgG1, IgG2a and IgG2b immunoglobulins from mouse serum using protein A-sepharose.
    Immunochemistry. 1978 Jul;15(7):429-36 PMID: 30693
  37. mu-Chains from a nonsecretor B cell line differ from secreted mu-chains at the C-terminal end.
    J Immunol. 1978 Dec;121(6):2435-9 PMID: 102695
  38. B lymphocytes contain three species of mu chains.
    Eur J Immunol. 1978 Dec;8(12):876-80 PMID: 103728
  39. Relationship between T-antigen and tumor-specific transplantation antigen in simian virus 40-transformed cells.
    J Virol. 1979 Jan;29(1):69-75 PMID: 219235
  40. Changes in surface immunoglobulin isotypes on purified antigen-binding cells after antigenic stimulation.
    J Immunol. 1979 May;122(5):2037-44 PMID: 376730
  41. Antigen-induced changes in the proportion of antigen-binding cells expressing IgM, IgG, and IgD receptors.
    J Immunol. 1979 Jun;122(6):2349-55 PMID: 109528
  42. B-cell influences on the induction of allotype suppressor T cells.
    J Exp Med. 1979 Jul 1;150(1):174-83 PMID: 312905
  43. RNA processing and the intervening sequence problem.
    Annu Rev Biochem. 1979;48:1035-69 PMID: 112912
  44. Structure of the constant and 3' untranslated regions of the murine gamma 2b heavy chain messenger RNA.
    Science. 1979 Dec 14;206(4424):1299-303 PMID: 117548
  45. Sequence of the cloned gene for the constant region of murine gamma 2b immunoglobulin heavy chain.
    Science. 1979 Dec 14;206(4424):1303-6 PMID: 117549
  46. Evidence for hydrophobic region within heavy chains of mouse B lymphocyte membrane-bound IgM.
    Proc Natl Acad Sci U S A. 1979 Nov;76(11):5515-9 PMID: 118450
  47. Localization of murine Ig-1b and Ig-1a (IgG 2a) allotypic determinants detected with monoclonal antibodies.
    Mol Immunol. 1979 Dec;16(12):1005-17 PMID: 94425
  48. Use of endo- and exoglycosidases for structural studies of glycoconjugates.
    Anal Biochem. 1979 Nov 15;100(1):1-14 PMID: 120683
  49. Memory B cells at successive stages of differentiation. Affinity maturation and the role of IgD receptors.
    J Exp Med. 1980 May 1;151(5):1071-87 PMID: 6966317
Article Info
Journal
The Journal of experimental medicine
Abbr.
J Exp Med
ISSN
0022-1007
DOI
10.1084/jem.151.5.1260
Published
1980-05-01
Pages
1260-74
Language
English
Region
United States
NLM ID
2985109R
PMCID
PMC2185862
Subset
IM
External Links
PubMed source DOI: 10.1084/jem.151.5.1260 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2