Home LiteratureArticle Details
PMID: 6151003 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Adjacent chromosomal regions can evolve at very different rates: evolution of the Drosophila 68C glue gene cluster.

Journal of molecular evolution ·Vol. 20 ·No. 3-4 ·1984-00-00 ·Pages 251-64

Meyerowitz EM, Martin CH

Abstract

The 68C puff is a highly transcribed region of the Drosophila melanogaster salivary gland polytene chromosomes. Three different classes of messenger RNA originate in a 5000-bp region in the puff; each class is translated to one of the salivary gland glue proteins sgs-3, sgs-7, or sgs-8. These messenger RNA classes are coordinately controlled, with each RNA appearing in the third larval instar and disappearing at the time of puparium formation. Their disappearance is initiated by the action of the steroid hormone ecdysterone. In the work reported here, we studied evolution of this hormone-regulated gene cluster in the melanogaster species subgroup of Drosophila. Genome blot hybridization experiments showed that five other species of this subgroup have DNA sequences that hybridize to D. melanogaster 68C sequences, and that these sequences are divided into a highly conserved region, which does not contain the glue genes, and an extraordinarily diverged region, which does. Molecular cloning of this DNA from D. simulans, D. erecta, D. yakuba, and D. teissieri confirmed the division of the region into a slowly and a rapidly evolving portion, and also showed that the rapidly evolving region of each species codes for third instar larval salivary gland RNAs homologous to the D. melanogaster glue mRNAs. The highly conserved region is at least 13,000 bp long, and is not known to code for any RNAs.

MeSH Terms
Animals Cloning, Molecular DNA Restriction Enzymes DNA, Recombinant/analysis Drosophila melanogaster/genetics Nucleic Acid Hybridization Phylogeny Poly A/genetics RNA, Messenger/genetics Salivary Proteins and Peptides/genetics Species Specificity
Chemicals
DNA, Recombinant RNA, Messenger Salivary Proteins and Peptides Poly A DNA Restriction Enzymes
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Meyerowitz E M
Martin C H
References (29)
29 references, click to expand
  1. Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I.
    J Mol Biol. 1977 Jun 15;113(1):237-51 PMID: 881736
  2. In vitro packaging of a lambda Dam vector containing EcoRI DNA fragments of Escherichia coli and phage P1.
    Gene. 1977 May;1(3-4):255-80 PMID: 338419
  3. A membrane-filter technique for the detection of complementary DNA.
    Biochem Biophys Res Commun. 1966 Jun 13;23(5):641-6 PMID: 5963888
  4. [RIBONUCLEIC ACID SYNTHESIS IN GIANT CHROMOSOMES. AUTORADIOGRAPHIC INVESTIGATIONS ON CHIRONOMUS TENTANS].
    Chromosoma. 1964 Apr 1;15:71-122 PMID: 14171171
  5. Sequential gene activation by ecdysone in polytene chromosomes of Drosophila melanogaster. II. The effects of inhibitors of protein synthesis.
    Dev Biol. 1974 Jul;39(1):141-57 PMID: 4209831
  6. Patterns of puffing activity in the salivary gland chromosomes of Drosophila. I. Autosomal puffing patterns in a laboratory stock of Drosophila melanogaster.
    Chromosoma. 1967;21(4):398-428 PMID: 6056758
  7. DNA sequences, gene regulation and modular protein evolution in the Drosophila 68C glue gene cluster.
    J Mol Biol. 1983 Aug 25;168(4):765-89 PMID: 6411930
  8. A novel arrangement of tandemly repeated genes at a major heat shock site in D. melanogaster.
    Cell. 1978 Aug;14(4):901-19 PMID: 99245
  9. Molecular weight estimation and separation of ribonucleic acid by electrophoresis in agarose-acrylamide composite gels.
    Biochemistry. 1968 Feb;7(2):668-74 PMID: 4868544
  10. Relationships within the melanogaster species subgroup of the genus Drosophila (Sophophora). II. Phylogenetic relationships between six species based upon polytene chromosome banding sequences.
    Proc R Soc Lond B Biol Sci. 1976 May 18;193(1112):275-94 PMID: 6967
  11. Estimating genetic divergence and genetic variability with restriction endonucleases.
    Proc Natl Acad Sci U S A. 1981 Oct;78(10):6329-33 PMID: 6273864
  12. Cosmids: a type of plasmid gene-cloning vector that is packageable in vitro in bacteriophage lambda heads.
    Proc Natl Acad Sci U S A. 1978 Sep;75(9):4242-6 PMID: 360212
  13. Sequential gene activation by ecdysone in polytene chromosomes of Drosophila melanogaster. I. Dependence upon ecdysone concentration.
    Dev Biol. 1973 Nov;35(1):47-61 PMID: 4207111
  14. Charon phages: safer derivatives of bacteriophage lambda for DNA cloning.
    Science. 1977 Apr 8;196(4286):161-9 PMID: 847462
  15. The structural genes for three Drosophila glue proteins reside at a single polytene chromosome puff locus.
    Mol Cell Biol. 1983 Apr;3(4):623-34 PMID: 6406838
  16. Localization of ecdysterone on polytene chromosomes of Drosophila melanogaster.
    Proc Natl Acad Sci U S A. 1980 Apr;77(4):2108-12 PMID: 6769118
  17. Detection of specific sequences among DNA fragments separated by gel electrophoresis.
    J Mol Biol. 1975 Nov 5;98(3):503-17 PMID: 1195397
  18. Mathematical model for studying genetic variation in terms of restriction endonucleases.
    Proc Natl Acad Sci U S A. 1979 Oct;76(10):5269-73 PMID: 291943
  19. Construction and characterization of new cloning vehicles. III. Derivatives of plasmid pBR322 carrying unique Eco RI sites for selection of Eco RI generated recombinant DNA molecules.
    Gene. 1978 Oct;4(2):121-36 PMID: 363519
  20. Sequential gene activation by ecdysone in polytene chromosomes of Drosophila melanogaster. III. Consequences of ecdysone withdrawal.
    Dev Biol. 1976 Dec;54(2):241-55 PMID: 825403
  21. Lambdoid phages that simplify the recovery of in vitro recombinants.
    Mol Gen Genet. 1977 Jan 7;150(1):53-61 PMID: 319344
  22. Patterns of puffing activity in the salivary gland chromosomes of Drosophila. VII. Homology of puffing patterns on chromosome arm 3L in D. melanogaster and D. yakuba, with notes on puffing in D. teissieri.
    Chromosoma. 1972;38(3):283-95 PMID: 4627364
  23. Analysis of restriction fragments of T7 DNA and determination of molecular weights by electrophoresis in neutral and alkaline gels.
    J Mol Biol. 1977 Feb 15;110(1):119-46 PMID: 845942
  24. Construction and characterization of new cloning vehicles. II. A multipurpose cloning system.
    Gene. 1977;2(2):95-113 PMID: 344137
  25. Genes of the major histocompatibility complex of the mouse.
    Annu Rev Immunol. 1983;1:529-68 PMID: 6152713
  26. Steroid regulation of RNAs transcribed from the Drosophila 68c polytene chromosome puff.
    Dev Biol. 1984 Mar;102(1):110-21 PMID: 6199239
  27. Molecular organization of a Drosophila puff site that responds to ecdysone.
    Cell. 1982 Jan;28(1):165-76 PMID: 6279311
  28. Sequencing end-labeled DNA with base-specific chemical cleavages.
    Methods Enzymol. 1980;65(1):499-560 PMID: 6246368
  29. Evolution of single-copy DNA and the ADH gene in seven drosophilids.
    J Mol Evol. 1982;19(1):62-71 PMID: 6761443
Article Info
Journal
Journal of molecular evolution
Abbr.
J Mol Evol
ISSN
0022-2844
DOI
10.1007/BF02104731
Published
1984-00-00
Pages
251-64
Language
English
Region
Germany
NLM ID
0360051
Subset
IM
Grants
NIGMS NIH HHS · GM28075 · United States
External Links
PubMed source DOI: 10.1007/BF02104731 View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2