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PMID: 6112752 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

gamma-Aminobutyric acid and benzodiazepine receptors: copurification and characterization.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 78 ·No. 3 ·1981-03-00 ·Pages 1939-42

Gavish M, Snyder SH

Abstract

gamma-Aminobutyric acid (GABA) and benzodiazepine receptors have been solubilized and purified by procedures such as gel filtration, ion-exchange, lectin, and affinity chromatographies. All of these procedures enhance the specific activity of each receptor to a similar extent. The drug specificities of [3H]muscimol and [3H]flunitrazepam binding sites are the same after extensive purification by affinity chromatography compared to the membrane bound and initially solubilized receptors. GABA and chloride stimulation of benzodiazepine binding is retained in pure receptors. Two bands are covalently labeled with [3H]flunitrazepam after ultraviolet irradiation of the purified receptor. The persistent association of GABA, benzodiazepine, and chloride recognition sites after extensive purification suggests that they may be part of a single macromolecular complex.

MeSH Terms
Animals Anti-Anxiety Agents/metabolism Binding, Competitive Brain/metabolism Cattle Flunitrazepam/metabolism Kinetics Receptors, Cell Surface/isolation & purification,metabolism Receptors, Drug/isolation & purification,metabolism Receptors, GABA-A gamma-Aminobutyric Acid/metabolism
Chemicals
Anti-Anxiety Agents Receptors, Cell Surface Receptors, Drug Receptors, GABA-A gamma-Aminobutyric Acid Flunitrazepam
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Gavish M
Snyder S H
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25 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.78.3.1939
Published
1981-03-00
Pages
1939-42
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC319251
Subset
IM
Grants
NIDA NIH HHS · DA-00266 · United States
NIMH NIH HHS · MH-18501 · United States
NINDS NIH HHS · NS-16375 · United States
External Links
PubMed source DOI: 10.1073/pnas.78.3.1939 PMC Full Text View journal details
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