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PMID: 6096823 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Optimizing selection of restriction enzymes in the search for DNA variants.

Nucleic acids research ·Vol. 12 ·No. 23 ·1984-12-11 ·Pages 9209-26

Wijsman EM

Abstract

A model is developed for predicting the relative efficiencies of different enzymes for detecting DNA variants when such variants are the result of single base-pair changes. 71 enzymes are analyzed for this ability in human DNA. Their relative ranked efficiencies are influenced by the sizes of the probes used, and the size of the smallest detectable fragment produced.

MeSH Terms
Base Sequence DNA/genetics DNA Restriction Enzymes/metabolism Genetic Variation Humans Kinetics Mathematics Models, Genetic Substrate Specificity
Chemicals
DNA DNA Restriction Enzymes
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Wijsman E M
References (35)
35 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/12.23.9209
Published
1984-12-11
Pages
9209-26
Language
English
Region
England
NLM ID
0411011
PMCID
PMC320450
Subset
IM
Grants
NIGMS NIH HHS · GM 28428 · United States
External Links
PubMed source DOI: 10.1093/nar/12.23.9209 PMC Full Text View journal details
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