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PMID: 6096808 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Structure of the spinach chloroplast genes for the D2 and 44 kd reaction-centre proteins of photosystem II and for tRNASer (UGA).

Nucleic acids research ·Vol. 12 ·No. 23 ·1984-12-11 ·Pages 8819-34

Holschuh K, Bottomley W, Whitfeld PR

Abstract

We have determined the sequence of the spinach (Spinacia oleracea) chloroplast genes for the photosystem II proteins, D2 and the 44 kd reaction-centre, chlorophyll a-binding protein, and for tRNASer (UGA). The 3' end of the D2 gene overlaps the first 50 bp of the 5' end of the gene for the 44 kd protein. Northern RNA hybridization analysis indicates the two genes are cotranscribed into a single 3.5 kb RNA. The predicted molecular weight of the 353-residue D2 protein is 39536 and that of the 473-residue 44 kd protein is 51816. Both proteins are hydrophobic containing at least five possible membrane-spanning domains. D2 shows significant homology to the 32 kd herbicide-binding protein (Zurawski et al., (1982) Proc. Natl. Acad. Sci. USA 79, 7699-7703), and parts of the 44 kd protein show obvious similarities to parts of the 51 kd reaction-centre, chlorophyll a-binding protein of photosystem II (Morris and Herrmann (1984) Nucleic Acids Res. 12, 2837-2850). The gene for tRNASer (UGA) which is on the opposite strand to and transcribed towards the photosystem II genes is 72% homologous with the corresponding Escherichia coli tRNASer.

MeSH Terms
Amino Acid Sequence Base Sequence Chlamydomonas/genetics Chlorophyll/genetics DNA Restriction Enzymes Genes Light-Harvesting Protein Complexes Nucleic Acid Hybridization Photosynthesis Photosynthetic Reaction Center Complex Proteins Photosystem II Protein Complex Plant Proteins/genetics Plants/genetics,metabolism Plasmids RNA, Transfer, Amino Acyl/genetics Species Specificity
Chemicals
Light-Harvesting Protein Complexes Photosynthetic Reaction Center Complex Proteins Photosystem II Protein Complex Plant Proteins RNA, Transfer, Amino Acyl Chlorophyll DNA Restriction Enzymes
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Holschuh K
Bottomley W
Whitfeld P R
References (32)
32 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/12.23.8819
Published
1984-12-11
Pages
8819-34
Language
English
Region
England
NLM ID
0411011
PMCID
PMC320421
Subset
IM
Databases
GENBANK
X01724
External Links
PubMed source DOI: 10.1093/nar/12.23.8819 PMC Full Text View journal details
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