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PMID: 6092842 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

DNA amplification and an unstable arginine gene in Streptomyces lividans 66.

Molecular & general genetics : MGG ·Vol. 195 ·No. 1-2 ·1984-00-00 ·Pages 134-8

Altenbuchner J, Cullum J

Abstract

Streptomyces lividans 66 produced spontaneous chloramphenicol-sensitive mutants (CmlS) at a frequency of about 1% of spores. The CmlS mutant strains were very unstable, giving Arg- mutants at frequencies of about 25% of spores. All the Arg- mutants had amplified a particular 5.75 kb DNA fragment into tandem repeats of 250-500 copies per chromosome.

MeSH Terms
Anti-Bacterial Agents/toxicity Arginine/metabolism Base Sequence DNA Restriction Enzymes DNA, Bacterial/genetics Drug Resistance, Microbial Gene Amplification Genes Genes, Bacterial Mutation Streptomyces/drug effects,genetics
Chemicals
Anti-Bacterial Agents DNA, Bacterial Arginine DNA Restriction Enzymes
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Altenbuchner J
Cullum J
References (22)
22 references, click to expand
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00332735
Published
1984-00-00
Pages
134-8
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00332735
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