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PMID: 6092510 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Interleukin 2 regulates the expression of Tac antigen on peripheral blood T lymphocytes.

The Journal of experimental medicine ·Vol. 160 ·No. 5 ·1984-11-01 ·Pages 1390-403

Welte K, Andreeff M, Platzer E, Holloway K, Rubin BY, Moore MA, Mertelsmann R

Abstract

We investigated the effect of OKT3 antibody and interleukin 2 (IL-2) on Tac antigen expression and the proliferation of human peripheral blood mononuclear leukocytes. OKT3 monoclonal antibody at low, nonmitogenic concentrations (25 pg/ml) or IL-2 alone at optimal concentrations (20 U/ml) did not induce IL-2 receptor expression, as measured by Tac antibody or by T cell proliferation. However, costimulation with these concentrations of OKT3 antibody and IL-2 led to Tac antigen expression and T cell proliferation. These data suggest that the T cells are activated in two steps: OKT3 antibody at 25 pg/ml does not induce Tac antigen expression, but preactivates T cells to become responsive to IL-2. The addition of exogenous IL-2 then leads to expression of the IL-2 receptor, as recognized by Tac antibody, and to subsequent proliferation.

MeSH Terms
Antibodies, Monoclonal/physiology Antigens, Surface/analysis,immunology DNA/analysis,biosynthesis Fluorescent Antibody Technique Humans Interferon-gamma/physiology Interleukin-2/biosynthesis,metabolism,physiology Kinetics Lymphocyte Activation Macrophage Activation Mitomycin Mitomycins/pharmacology Protein Biosynthesis T-Lymphocytes/analysis,immunology Tumor Necrosis Factor Receptor Superfamily, Member 7
Chemicals
Antibodies, Monoclonal Antigens, Surface Interleukin-2 Mitomycins Tumor Necrosis Factor Receptor Superfamily, Member 7 Mitomycin Interferon-gamma DNA
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Welte K
Andreeff M
Platzer E
Holloway K
Rubin B Y
Moore M A
Mertelsmann R
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Article Info
Journal
The Journal of experimental medicine
Abbr.
J Exp Med
ISSN
0022-1007
DOI
10.1084/jem.160.5.1390
Published
1984-11-01
Pages
1390-403
Language
English
Region
United States
NLM ID
2985109R
PMCID
PMC2187497
Subset
IM
Grants
NCI NIH HHS · CA-20194 · United States
NCI NIH HHS · CA-33873 · United States
NCI NIH HHS · CA-34995 · United States
External Links
PubMed source DOI: 10.1084/jem.160.5.1390 PMC Full Text View journal details
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