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PMID: 6091041 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Introns and their flanking sequences of Bombyx mori rDNA.

Nucleic acids research ·Vol. 12 ·No. 17 ·1984-09-11 ·Pages 6861-9

Fujiwara H, Ogura T, Takada N, Miyajima N, Ishikawa H, Maekawa H

Abstract

We obtained two different clones (16 kb and 13 kb) of B. mori rDNA with intron sequence within the 28S-rRNA coding region. The sequence surrounding the intron was found to be highly conserved as indicated in several eukaryotes (Tetrahymena, Drosophila and Xenopus). The 28S rRNA-coding sequence of 16 kb and 13 kb clone was interrupted at precisely the same sites as those where the D. melanogaster rDNA interrupted by the type I and type II intron, respectively. The intron sequences of B. mori were different from those of D. melanogaster. In 16 kb clone, the intron was flanked by 14 bp duplication of the junction sequence, which was also present once within the 28S rRNA-coding region of rDNA without intron. This 14 bp sequence was identical with those surrounding the introns of Dipteran rDNAs.

MeSH Terms
Animals Base Sequence Bombyx/genetics Cloning, Molecular DNA Restriction Enzymes DNA, Ribosomal/genetics Drosophila melanogaster/genetics Molecular Weight RNA, Ribosomal/genetics Species Specificity Xenopus
Chemicals
DNA, Ribosomal RNA, Ribosomal DNA Restriction Enzymes
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Fujiwara H
Ogura T
Takada N
Miyajima N
Ishikawa H
Maekawa H
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30 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/12.17.6861
Published
1984-09-11
Pages
6861-9
Language
English
Region
England
NLM ID
0411011
PMCID
PMC320122
Subset
IM
Databases
GENBANK
X01049, X01050, X01051, X01052
External Links
PubMed source DOI: 10.1093/nar/12.17.6861 PMC Full Text View journal details
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