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PMID: 6088794 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Genetic variation among lentiviruses: homology between visna virus and caprine arthritis-encephalitis virus is confined to the 5' gag-pol region and a small portion of the env gene.

Journal of virology ·Vol. 51 ·No. 3 ·1984-09-00 ·Pages 713-21

Pyper JM, Clements JE, Molineaux SM, Narayan O

Abstract

Visna virus of sheep and arthritis-encephalitis virus of goats are serologically related but genetically distinct retroviruses which cause slowly progressive diseases in their natural hosts. To localize homologous regions of the DNAs of these two viruses, we constructed a physical map of caprine arthritis-encephalitis virus DNA and aligned it with the viral RNA. Cloned probes of visna virus DNA were then used to localize regions of homology with the caprine arthritis-encephalitis virus DNA. These studies showed homology in the 5' region of the genome encompassing U5 and the gag and pol genes and also in a small region in the env gene. These findings correlate with biological data suggesting that the regions of the DNA which are homologous may be responsible for virus group characteristics such as the closely related virus core antigens. Regions which did not show homology such as large sections in the env gene may represent unique sequences which control highly strain-specific characteristics such as the neutralization antigen and specific cell tropisms.

MeSH Terms
Animals Antigens, Viral/genetics Cells, Cultured Cloning, Molecular DNA Restriction Enzymes Gene Products, gag Genes Genes, Viral Genetic Variation Goats Nucleic Acid Hybridization Retroviridae/genetics Sheep Species Specificity Viral Proteins/genetics,isolation & purification Visna-maedi virus/genetics
Chemicals
Antigens, Viral Gene Products, gag Viral Proteins DNA Restriction Enzymes
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Pyper J M
Clements J E
Molineaux S M
Narayan O
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43 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.51.3.713-721.1984
Published
1984-09-00
Pages
713-21
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC255836
Subset
IM
Grants
NINDS NIH HHS · NS 16145 · United States
NINDS NIH HHS · P01-NS-15721 · United States
External Links
PubMed source DOI: 10.1128/JVI.51.3.713-721.1984 PMC Full Text View journal details
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