Home LiteratureArticle Details
PMID: 6087338 Published · ppublish English Journal Article

Alpha-factor-directed synthesis and secretion of mature foreign proteins in Saccharomyces cerevisiae.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 81 ·No. 15 ·1984-08-00 ·Pages 4642-6

Brake AJ, Merryweather JP, Coit DG, Heberlein UA, Masiarz FR, Mullenbach GT, Urdea MS, Valenzuela P, Barr PJ

Abstract

Saccharomyces cerevisiae cells were transformed with plasmids containing hybrid genes in which the sequence encoding mature human epidermal growth factor was joined to sequences encoding the leader region (preprosegment) of the precursor of the yeast mating pheromone alpha-factor. These cells accurately process the hybrid protein and efficiently secrete authentic biologically active human epidermal growth factor into the medium.

MeSH Terms
Amino Acid Sequence Base Sequence Cloning, Molecular/methods DNA, Recombinant Epidermal Growth Factor/genetics,metabolism Gene Expression Regulation Genetic Vectors Mating Factor Mutation Peptides/genetics Plasmids Protein Processing, Post-Translational Saccharomyces cerevisiae/genetics
Chemicals
DNA, Recombinant Peptides Mating Factor Epidermal Growth Factor
Authors & Affiliations
9 authors, click to expand affiliations / ORCID
Brake A J
Merryweather J P
Coit D G
Heberlein U A
Masiarz F R
Mullenbach G T
Urdea M S
Valenzuela P
Barr P J
References (24)
24 references, click to expand
  1. A protein sequenator.
    Eur J Biochem. 1967 Mar;1(1):80-91 PMID: 6059350
  2. Secretion of human interferons by yeast.
    Science. 1983 Feb 11;219(4585):620-5 PMID: 6186023
  3. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
    Nature. 1970 Aug 15;227(5259):680-5 PMID: 5432063
  4. Human epidermal growth factor: isolation and chemical and biological properties.
    Proc Natl Acad Sci U S A. 1975 Apr;72(4):1317-21 PMID: 1055407
  5. Construction and characterization of new cloning vehicles. II. A multipurpose cloning system.
    Gene. 1977;2(2):95-113 PMID: 344137
  6. Transformation of yeast.
    Proc Natl Acad Sci U S A. 1978 Apr;75(4):1929-33 PMID: 347451
  7. Transformation of yeast by a replicating hybrid plasmid.
    Nature. 1978 Sep 14;275(5676):104-9 PMID: 357984
  8. Epidermal growth factor.
    Annu Rev Biochem. 1979;48:193-216 PMID: 382984
  9. Stepwise cleavage of the pro part of promelittin by dipeptidylpeptidase IV. Evidence for a new type of precursor--product conversion.
    Eur J Biochem. 1980 Oct;111(1):49-58 PMID: 7002560
  10. Cloning of the yeast methionyl-tRNA synthetase gene.
    J Biol Chem. 1981 Mar 10;256(5):2324-8 PMID: 6257714
  11. A gas-liquid solid phase peptide and protein sequenator.
    J Biol Chem. 1981 Aug 10;256(15):7990-7 PMID: 7263636
  12. Rapid and efficient cosmid cloning.
    Nucleic Acids Res. 1981 Jul 10;9(13):2989-98 PMID: 6269067
  13. Order of events in the yeast secretory pathway.
    Cell. 1981 Aug;25(2):461-9 PMID: 7026045
  14. Distinct repressible mRNAs for cytoplasmic and secreted yeast invertase are encoded by a single gene.
    Cell. 1981 Aug;25(2):525-36 PMID: 7026048
  15. An 'internal' signal sequence directs secretion and processing or proinsulin in bacteria.
    Nature. 1981 Nov 12;294(5837):176-8 PMID: 7029306
  16. Two differentially regulated mRNAs with different 5' ends encode secreted with intracellular forms of yeast invertase.
    Cell. 1982 Jan;28(1):145-54 PMID: 7039847
  17. Structure of a yeast pheromone gene (MF alpha): a putative alpha-factor precursor contains four tandem copies of mature alpha-factor.
    Cell. 1982 Oct;30(3):933-43 PMID: 6754095
  18. Yeast alpha factor is processed from a larger precursor polypeptide: the essential role of a membrane-bound dipeptidyl aminopeptidase.
    Cell. 1983 Mar;32(3):839-52 PMID: 6339075
  19. A functional prepro-alpha-factor gene in Saccharomyces yeasts can contain three, four, or five repeats of the mature pheromone sequence.
    Mol Cell Biol. 1983 Aug;3(8):1440-50 PMID: 6353204
  20. An MF alpha 1-SUC2 (alpha-factor-invertase) gene fusion for study of protein localization and gene expression in yeast.
    Proc Natl Acad Sci U S A. 1983 Dec;80(23):7080-4 PMID: 6359161
  21. Glycosylation and processing of prepro-alpha-factor through the yeast secretory pathway.
    Cell. 1984 Feb;36(2):309-18 PMID: 6420074
  22. Chemical synthesis of a gene for human epidermal growth factor urogastrone and its expression in yeast.
    Proc Natl Acad Sci U S A. 1983 Dec;80(24):7461-5 PMID: 6369317
  23. Oligonucleotide-directed mutagenesis using M13-derived vectors: an efficient and general procedure for the production of point mutations in any fragment of DNA.
    Nucleic Acids Res. 1982 Oct 25;10(20):6487-500 PMID: 6757864
  24. A complementation analysis of the restriction and modification of DNA in Escherichia coli.
    J Mol Biol. 1969 May 14;41(3):459-72 PMID: 4896022
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.81.15.4642
Published
1984-08-00
Pages
4642-6
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC391546
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.81.15.4642 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2