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PMID: 6087295 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Human purine nucleoside phosphorylase cDNA sequence and genomic clone characterization.

Nucleic acids research ·Vol. 12 ·No. 14 ·1984-07-25 ·Pages 5779-87

Williams SR, Goddard JM, Martin DW

Abstract

The isolation of a cDNA clone containing the complete coding region for human purine nucleoside phosphorylase (PNP) has been described previously. In this report we present the nucleotide sequence of this cDNA clone and compare the derived amino acid sequence, encoding a protein of 32 kilodaltons, with the published amino acid composition. Using a fragment of the cDNA clone as a probe, human PNP genomic clones from a bacteriophage lambda library have been isolated and the structural organization of the wild type PNP gene determined.

MeSH Terms
Base Sequence Chromosome Mapping Cloning, Molecular DNA/genetics DNA Restriction Enzymes Genes Humans Pentosyltransferases/genetics Purine-Nucleoside Phosphorylase/genetics
Chemicals
DNA Pentosyltransferases Purine-Nucleoside Phosphorylase DNA Restriction Enzymes
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Williams S R
Goddard J M
Martin D W
References (28)
28 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/12.14.5779
Published
1984-07-25
Pages
5779-87
Language
English
Region
England
NLM ID
0411011
PMCID
PMC320030
Subset
IM
Grants
NIAID NIH HHS · AI-17435 · United States
Databases
GENBANK
K02574
External Links
PubMed source DOI: 10.1093/nar/12.14.5779 PMC Full Text View journal details
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