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PMID: 6087274 Published · ppublish English Journal Article

Alteration of apparent restriction endonuclease recognition specificities by DNA methylases.

Nucleic acids research ·Vol. 12 ·No. 13 ·1984-07-11 ·Pages 5165-73

Nelson M, Christ C, Schildkraut I

Abstract

An in vitro method of altering the apparent cleavage specificities of restriction endonucleases was developed using DNA modification methylases. This method was used to reduce the number of cleavage sites for 34 restriction endonucleases. In particular, single-site cleavages were achieved for Nhe I in Adeno-2 DNA and for Acc I and Hinc II in pBR322 DNA by specifically methylating all but one recognition sequence.

MeSH Terms
Base Composition Base Sequence DNA DNA (Cytosine-5-)-Methyltransferases/metabolism DNA Restriction Enzymes/metabolism Methyltransferases/metabolism Molecular Weight Substrate Specificity
Chemicals
DNA Methyltransferases DNA (Cytosine-5-)-Methyltransferases DNA Restriction Enzymes
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Nelson M
Christ C
Schildkraut I
References (8)
8 references, click to expand
  1. Letter: A suggested nomenclature for bacterial host modification and restriction systems and their enzymes.
    J Mol Biol. 1973 Dec 15;81(3):419-23 PMID: 4588280
  2. A cautionary note on the use of certain restriction endonucleases with methylated substrates.
    Gene. 1980 Oct;11(1-2):169-71 PMID: 6254852
  3. Restriction enzyme digestion of hemimethylated DNA.
    Nucleic Acids Res. 1981 Jun 11;9(11):2509-15 PMID: 6269052
  4. Restriction and modification enzymes and their recognition sequences.
    Nucleic Acids Res. 1984;12 Suppl:r167-204 PMID: 6328451
  5. Purification and characterization of two new modification methylases: MClaI from Caryophanon latum L and MTaqI from Thermus aquaticus YTI.
    Nucleic Acids Res. 1981 Dec 21;9(24):6795-804 PMID: 6278447
  6. Restriction and modification in Bacillus subtilis: DNA methylation potential of the related bacteriophages Z, SPR, SP beta, phi 3T, and rho 11.
    J Virol. 1983 May;46(2):446-53 PMID: 6302313
  7. The effect of site specific methylation on restriction endonuclease cleavage (update).
    Nucleic Acids Res. 1983 Jan 11;11(1):r169-73 PMID: 6306558
  8. Modification profiles of bacterial genomes.
    Nucleic Acids Res. 1982 Feb 11;10(3):913-34 PMID: 6278441
Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/12.13.5165
Published
1984-07-11
Pages
5165-73
Language
English
Region
England
NLM ID
0411011
PMCID
PMC318911
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/12.13.5165 PMC Full Text View journal details
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