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PMID: 6086583 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Cloning and expression of a gene segment encoding the enzymatic moiety of Pseudomonas aeruginosa exotoxin A.

Journal of bacteriology ·Vol. 159 ·No. 2 ·1984-08-00 ·Pages 683-7

Mozola MA, Wilson RB, Jordan EM, Draper RK, Clowes RC

Abstract

Using the broad-host-range plasmid vector pRO1614, we cloned a segment of the gene from Pseudomonas aeruginosa PA103 encoding the enzymatically active part of the exotoxin A protein. Expression of the cloned gene segment has been achieved both in Escherichia coli and in a nontoxigenic P. aeruginosa host, as assayed by the production of exotoxin A-related antigen and by the ability of the gene product to ADP-ribosylate elongation factor 2. Western blot hybridization analysis revealed a series of polypeptides antigenically related to exotoxin A, the largest of which had a molecular weight of ca. 50,000.

MeSH Terms
ADP Ribose Transferases Adenosine Diphosphate Ribose/metabolism Bacterial Toxins Base Sequence Cloning, Molecular DNA Restriction Enzymes Exotoxins/genetics Genes Genes, Bacterial Nucleic Acid Hybridization Peptide Elongation Factor 2 Peptide Elongation Factors/metabolism Plasmids Pseudomonas aeruginosa/genetics Virulence Factors
Chemicals
Bacterial Toxins Exotoxins Peptide Elongation Factor 2 Peptide Elongation Factors Virulence Factors Adenosine Diphosphate Ribose ADP Ribose Transferases toxA protein, Pseudomonas aeruginosa DNA Restriction Enzymes
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Mozola M A
Wilson R B
Jordan E M
Draper R K
Clowes R C
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36 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.159.2.683-687.1984
Published
1984-08-00
Pages
683-7
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC215698
Subset
IM
Grants
NIAID NIH HHS · AI-10638 · United States
NIAID NIH HHS · AI-17400 · United States
External Links
PubMed source DOI: 10.1128/jb.159.2.683-687.1984 PMC Full Text View journal details
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