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PMID: 6032987 Published · ppublish English Journal Article

Physiology of rat-liver polysomes. The stability of messenger ribonucleic acid and ribosomes.

The Biochemical journal ·Vol. 103 ·No. 2 ·1967-05-00 ·Pages 556-66

Wilson SH, Hoagland MB

Abstract

Starvation of rats for several days led to marked decrease in cytoplasmic polysomes and accumulation of breakdown products having S values less than 200s. Re-feeding of the starved animals induced a rapid reassembly of polysomes. These newly formed polysomes, in the presence of actinomycin D, decayed in a biphasic fashion: about two-thirds decayed with an apparent half-life of 3-3(1/2)hr. but the other one-third were much more stable. Evidence that polysome decay is an accurate reflexion of messenger RNA stability is presented, and it is concluded that in the presence of large doses of actinomycin D, rat-liver cytoplasm contains messenger RNA classes of widely varying stability, the more stable class having a half-life of at least 80hr. The half-life of liver ribosomes was also determined and was found to be 110-127hr.

MeSH Terms
Animals Carbon Isotopes Centrifugation, Density Gradient Chemical Precipitation Chromosomes Cytoplasm/analysis Dactinomycin/pharmacology Diet Female Injections, Intraperitoneal Leucine/pharmacology Liver/cytology Orotic Acid Protein Biosynthesis RNA, Messenger Rats Ribosomes Spectrophotometry Starvation Trichloroacetic Acid Ultracentrifugation
Chemicals
Carbon Isotopes RNA, Messenger Dactinomycin Trichloroacetic Acid Orotic Acid Leucine
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Wilson S H
Hoagland M B
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Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj1030556
Published
1967-05-00
Pages
556-66
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1270442
Subset
IM
External Links
PubMed source DOI: 10.1042/bj1030556 PMC Full Text View journal details
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