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PMID: 596935 Published · ppublish English Journal Article

Biochemical characterization of connective tissue macromolecules derived from cutaneous fibrosarcoma.

Archives for dermatological research = Archiv fur dermatologische Forschung ·Vol. 260 ·No. 2 ·1977-12-12 ·Pages 93-102

Matsubayashi S, Shinkai H, Sano S

Abstract

Tumor tissue of cutaneous fibrosarcoma was solubilized with salt, acetic acid and salt-extracted after pepsin treatment. Type III collagen was observed in neutral soluble collagen, 1.5 M and 2.4 M NaCl precipitated fractions after pepsin treatment. Type III collagen fraction precipitated with 1.5 M NaCl eluted in the alpha2 region when chromatographed on CM-cellulose without reduction, while the type III collagen partially purified with 1.5 M NaCl eluted between the position of alpha1(I) and beta12 after reduction and alkylation. Analysis of amino acid showed the presence of cysteine and the high content of hydroxyproline following CM-chromatography of 1.5 M NaCl precipitated type III collagen fraction. Acidic glycosaminoglycans were composed of hyaluronic acid, chondroitin sulfate, dermatan sulfate and heparan sulfate, and the first two were major components of fibrosarcoma.

MeSH Terms
Aged Collagen/analysis Connective Tissue/analysis,pathology Female Fibrosarcoma/analysis,pathology Histocytochemistry Humans Skin Neoplasms/analysis,pathology
Chemicals
Collagen
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Matsubayashi S
Shinkai H
Sano S
References (19)
19 references, click to expand
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Article Info
Journal
Archives for dermatological research = Archiv fur dermatologische Forschung
Abbr.
Arch Dermatol Res
DOI
10.1007/BF00561114
Published
1977-12-12
Pages
93-102
Language
English
Region
Germany
NLM ID
7512589
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00561114
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