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PMID: 593886 Published · ppublish English Comparative Study Journal Article

Determination of the sequence homology between the four RNA species of cucumber mosaic virus by hybridization analysis with complementary DNA.

Nucleic acids research ·Vol. 4 ·No. 11 ·1977-11-00 ·Pages 3787-802

Gould AR, Symons RH

Abstract

The method of Taylor etal., (11) has been used to transcribe complementary DNA probes from the four major RNA species of cucumber mosaic virus (RNAs 1 - 4 in order of decreasing molecular weight). Analysis of the kinetics of hybridization of these probes in homologous and heterologous complementary DNA-RNA hybridization reactions has shown that the sequence of the smallest RNA (RNA 4), which contains the coat protein gene, is present within RNA 3. RNAs 1 and 2 are unique RNA molecules while each has a region of approximately 300 nucleotides in common with RNA 4.Images

MeSH Terms
Base Sequence DNA, Viral Kinetics Mosaic Viruses Nucleic Acid Hybridization Plant Viruses RNA, Viral
Chemicals
DNA, Viral RNA, Viral
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Gould A R
Symons R H
References (20)
20 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
Published
1977-11-00
Pages
3787-802
Language
English
Region
England
NLM ID
0411011
PMCID
PMC343200
Subset
IM
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