Comparison of two serologically distinct ribonucleic acid bacteriophages. I. Properties of the viral particles.

Journal of bacteriology ·Vol. 91 ·No. 1 ·1966-01-00 ·Pages 442-8

Overby LR, Barlow GH, Doi RH, Jacob M, Spiegelman S

Abstract

Overby, L. R. (University of Illinois, Urbana), G. H. Barlow, R. H. Doi, Monique Jacob, and S. Spiegelman. Comparison of two serologically distinct ribonucleic acid bacteriophages. I. Properties of the viral particle. J. Bacteriol. 91:442-448. 1966.-Two ribonucleic acid (RNA) coliphages, MS-2 and Qbeta, have been characterized physically and serologically. MS-2 has an S(20, w) value of 79, a molecular weight of 3.6 x 10(6), a density of 1.422, and pH 3.9 as its isoelectric point. Qbeta has an S(20, w) of 84, a molecular weight of 4.2 x 10(6), a density of 1.439, and an isoelectric point at pH 5.3. One host (Escherichia coli A-19) permits a distinction between the two on the basis of a marked difference in plaque size. They are distinct immunochemically, no serological cross-reaction being detectable.

MeSH Terms

Centrifugation Chemical Phenomena Chemistry, Physical Coliphages/immunology Electrophoresis In Vitro Techniques Molecular Weight Photometry RNA Viruses Spectrum Analysis

Authors & Affiliations

5 authors, click to expand affiliations / ORCID

Overby L R

Barlow G H

Doi R H

Jacob M

Spiegelman S

References (14)

14 references, click to expand

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Article Info

Journal

Journal of bacteriology

Abbr.

J Bacteriol

ISSN

0021-9193

DOI

10.1128/jb.91.1.442-448.1966

Published

1966-01-00

Pages

442-8

Language

English

Region

United States

NLM ID

2985120R

PMCID

PMC315966

Subset

External Links

PubMed source DOI: 10.1128/jb.91.1.442-448.1966 PMC Full Text View journal details

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Comparison of two serologically distinct ribonucleic acid bacteriophages. I. Properties of the viral particles.

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