DETERMINATION OF BASE RATIOS OF SIX RIBONUCLEIC ACID BACTERIOPHAGES SPECIFIC TO ESCHERICHIA COLI.

The Biochemical journal ·Vol. 95 ·1965-04-00 ·Pages 82-93

BISHOP DH, BRADLEY DE

Abstract

1. A method is described for the isolation of single-stranded-RNA coliphages. Two of the six RNA coliphages investigated were new strains. 2. The base ratios of six RNA coliphages were determined by labelling the host bacterium with [(32)P]-phosphate, purification of the radioactive coliphages and separation of 2',3'-ribonucleotides liberated by alkaline hydrolysis of the coliphage RNA. 3. All six of the coliphages were morphologically similar, contained single-stranded RNA, and had sedimentation coefficient 80+/-5s. 4. The six RNA coliphages fell into two distinct groups, both serologically and in terms of their RNA base ratios.

Keywords

ADENINE BIOCHEMISTRY CHROMATOGRAPHY COLIPHAGES CYTOSINE DEOXYRIBONUCLEASE ELECTROPHORESIS EXPERIMENTAL LAB STUDY GUANINE ION EXCHANGE RESINS NUCLEOTIDES RIBONUCLEASE RIBOSOMES RNA VIRAL URACIL

MeSH Terms

Adenine Bacteriophages Base Composition Biochemical Phenomena Biochemistry Chromatography Coliphages Cytosine DNA Deoxyribonucleases Electrophoresis Escherichia coli Guanine Hydrolysis Ion Exchange Resins Nucleotides RNA RNA, Viral Research Ribonucleases Ribosomes Uracil

Chemicals

Ion Exchange Resins Nucleotides RNA, Viral Uracil Guanine RNA Cytosine DNA Deoxyribonucleases Ribonucleases Adenine

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

BISHOP D H

BRADLEY D E

References (10)

10 references, click to expand

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Article Info

Journal

The Biochemical journal

Abbr.

Biochem J

ISSN

0264-6021

DOI

10.1042/bj0950082

Published

1965-04-00

Pages

82-93

Language

English

Region

England

NLM ID

2984726R

PMCID

PMC1215180

Subset

External Links

PubMed source DOI: 10.1042/bj0950082 PMC Full Text View journal details

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DETERMINATION OF BASE RATIOS OF SIX RIBONUCLEIC ACID BACTERIOPHAGES SPECIFIC TO ESCHERICHIA COLI.

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