The effect of matrix on the binding of albumin to immobilized Cibacron Blue.

The Biochemical journal ·Vol. 167 ·No. 1 ·1977-10-01 ·Pages 301-3

Angal S, Dean PD

Abstract

With Cibacron Blue as a ligand, the suitability of Sepharose, Sephacryl, cellulose and Ultrogel as column supports for the affinity chromatography of human serum albumin was examined. Sepharose and Sephacryl were the most effective matrices. Albumin was readily removed from such columns by using an electrophoretic desorption technique.

MeSH Terms

Chromatography, Affinity/methods Coloring Agents Humans Serum Albumin/isolation & purification Triazines

Chemicals

Coloring Agents Serum Albumin Triazines

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Angal S

Dean P D

References (14)

14 references, click to expand

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Article Info

Journal

The Biochemical journal

Abbr.

Biochem J

ISSN

0264-6021

DOI

10.1042/bj1670301

Published

1977-10-01

Pages

301-3

Language

English

Region

England

NLM ID

2984726R

PMCID

PMC1183653

Subset

External Links

PubMed source DOI: 10.1042/bj1670301 PMC Full Text View journal details

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The effect of matrix on the binding of albumin to immobilized Cibacron Blue.

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