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PMID: 568182 Published · ppublish English Journal Article

Biochemical studies on bovine adenovirus type 3. III. Cleavage maps of viral DNA by restriction endoncleases EcoRI, BamHI, and HindIII.

Journal of virology ·Vol. 28 ·No. 1 ·1978-10-00 ·Pages 212-8

Kurokawa T, Igarashi K, Sugino Y

Abstract

Cleavage of bovine adenovirus type 3 (BAV3) DNA by restriction endonucleases EcoRI, BamHI, and HindIII yielded 7 (A to G), 5 (A to E), and 12 (A to L) fragments, respectively. The order of these fragments has been determined to be GDACBFE for EcoRI fragments, AEBDC for BamHI fragments, and JEBKACDHFGIL for HindIII fragments, and cleavage sites of these enzymes have been mapped on the genome of BAV3. BAV3 preparation contains incomplete virus whose genome has a deletion of about 13% of complete virus genome. Restriction endonuclease digestion of the incomplete virus DNA revealed that EcoRI E and F, BamHI C and HindIII G, I, and L fragments were deleted. Therefore, the deleted region of incomplete virus DNA is located near the right-hand end of the BAV3 DNA molecule, a result consistent with our previous electron-microscopic observations on heteroduplex molecules formed between complete and incomplete BAV3 DNA.

MeSH Terms
Adenoviridae/analysis,genetics Animals Cattle Cell Line DNA Restriction Enzymes/metabolism DNA, Viral/analysis Genes, Viral Molecular Weight
Chemicals
DNA, Viral DNA Restriction Enzymes
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Kurokawa T
Igarashi K
Sugino Y
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21 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.28.1.212-218.1978
Published
1978-10-00
Pages
212-8
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC354260
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.28.1.212-218.1978 PMC Full Text View journal details
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