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PMID: 1171993 Published · ppublish English Journal Article

Biochemical studies on bovine adenovirus type 3. I. Purification and properties.

Journal of virology ·Vol. 16 ·No. 3 ·1975-09-00 ·Pages 621-33

Niiyama Y, Igarashi K, Tsukamoto K, Kurokawa T, Sugino Y

Abstract

Bovine adenovirus type 3 (BAV3) was purified and its properties were studied. On productive infection of CKT1 cells (a cell line derived from calf kidney) with BAV3, it was observed that viral DNA synthesis was initiated after about 24 h and its rate was maximal after about 40 h. Maturation of the virus occurred several hours after this. Purified BAV3 was separated into four discrete bands by CsCl density gradient centrifugation (complete, incomplete, empty, and degraded viruses). The complete BAV3 was similar in size and structure to human and avian adenoviruses. Polyacrylamide gel electrophoresis showed that the complete BAV3 virion contained at least 10 polypeptides. The total structural proteins of the virion had a similar amino acid composition to those of human adenoviruses. DNA of the complete virus was a linear duplex and its contour length was 12.3 +/- 0.9 mum. The So20,w value of the DNA was 32.9S and its buoyant density in CsCl was 1.717 g/ml. There was about 25% homology between the DNAs of BAV3 and human adenovirus type 5 by filter hybridization. It was also noted that BAV3 produced incomplete virus. The incomplete virus was similar in morphology to the complete virus and contained almost all the structural polypeptides of the latter, but lacked infectivity. However, its DNA had a deletion(s) (13%) which seemed to locate near a terminal.

MeSH Terms
Adenoviridae/analysis,isolation & purification,ultrastructure Animals Cattle Cell Line Cytosine/analysis DNA, Circular/analysis DNA, Viral/analysis,biosynthesis Guanine/analysis Kinetics Peptides/analysis Viral Proteins/analysis
Chemicals
DNA, Circular DNA, Viral Peptides Viral Proteins Guanine Cytosine
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Niiyama Y
Igarashi K
Tsukamoto K
Kurokawa T
Sugino Y
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43 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.16.3.621-633.1975
Published
1975-09-00
Pages
621-33
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC354710
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.16.3.621-633.1975 PMC Full Text View journal details
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