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PMID: 5665 Published · ppublish English Journal Article

Characterisation of eukaryotic ribosomal proteins.

Molecular & general genetics : MGG ·Vol. 142 ·No. 4 ·1976-12-31 ·Pages 299-316

Martini OH, Gould HJ

Abstract

A simple method of two-dimensional polyacrylamide gel electrophoresis is described which affords: (1) high resolution of eukaryotic ribosomal proteins; (2) good recovery of protein in the transfer from first to second dimension; and (3) characterisation of the separated proteins in terms of molecular weights and other electrophoretic properties. Using this method, we have characterised 70 proteins in rabbit reticulocyte ribosomes, 30 from the small subunit and 40 from the large subunit. The molecular weight distribution is compared with those obtained by other authors after fractionation of the proteins in two dimensions.

MeSH Terms
Animals Electrophoresis, Polyacrylamide Gel/methods Hydrogen-Ion Concentration Molecular Weight Rabbits Reticulocytes/analysis Ribosomal Proteins/analysis
Chemicals
Ribosomal Proteins
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Martini O H
Gould H J
References (45)
45 references, click to expand
  1. Ribosomal aggregate engaged in protein synthesis: characterization of the ergosome.
    Nature. 1963 Feb 2;197:430-5 PMID: 14000153
  2. Purification and characterization of 50S ribosomal proteins of Escherichia coli.
    Mol Gen Genet. 1971;112(3):229-42 PMID: 4942360
  3. The identification of the eukaryotic ribosomal proteins homologous with Escherichia coli proteins L7 and L12.
    Proc Natl Acad Sci U S A. 1974 Dec;71(12):4723-6 PMID: 4140509
  4. Further observations on the polynucleotide-induced stimulation of protein synthesis by cell-free preparations from rabbit reticulocytes.
    Biochem J. 1965 Nov;97(2):595-606 PMID: 16749170
  5. Determination of the number of proteins in liver ribosomes and ribosomal subunits by two-dimensional polyacrylamide gel electrophoresis.
    J Biol Chem. 1972 Jul 25;247(14):4460-7 PMID: 5043849
  6. Studies on proteins of animal ribosomes. 8. Two-dimensional polyacrylamide gel electrophoresis of ribosomal proteins of rat liver.
    Biochim Biophys Acta. 1971 Sep 28;243(3):416-9 PMID: 5001984
  7. Molecular weight estimation of polypeptide chains by electrophoresis in SDS-polyacrylamide gels.
    Biochem Biophys Res Commun. 1967 Sep 7;28(5):815-20 PMID: 4861258
  8. Quantitation of eukaryotic ribosomal proteins separated by two-dimensional polyacrylamide gel electrophoresis.
    FEBS Lett. 1975 Aug 15;56(2):205-11 PMID: 1157940
  9. Studies on proteins of animal ribosomes. 13. Enumeration of ribosomal proteins of rat liver.
    FEBS Lett. 1972 Oct 1;26(1):228-32 PMID: 4636733
  10. 80S-ribosomal proteins.
    Biochem Soc Symp. 1973;37(0):51-68 PMID: 4605464
  11. Phosphorylation of rabbit reticulocyte ribosomal proteins in vitro.
    Biochim Biophys Acta. 1973 Feb 21;295(2):621-9 PMID: 4699579
  12. THE CHROMOSOMAL SITE SPECIFYING A RIBOSOMAL PROTEIN IN ESCHERICHIA COLI.
    Proc Natl Acad Sci U S A. 1964 Dec;52:1367-74 PMID: 14243510
  13. Formation of active hybrids from subunits of muscle ribosomes from normal and diabetic rats.
    Proc Natl Acad Sci U S A. 1968 Jun;60(2):569-74 PMID: 5248814
  14. Characterization of the proteins of the small subunits of rat liver ribosomes.
    Biochim Biophys Acta. 1972 Dec 28;285(2):473-82 PMID: 4633644
  15. Disk electrophoresis of basic proteins and peptides on polyacrylamide gels.
    Nature. 1962 Jul 21;195:281-3 PMID: 14491328
  16. Correlation of 30S ribosomal proteins of Escherichia coli isolated in different laboratories.
    Mol Gen Genet. 1971;111(4):327-33 PMID: 4998702
  17. Studies on rabbit reticulocyte ribosomes, II. Separation of the ribosomal proteins by two-dimensional electrophoresis.
    Hoppe Seylers Z Physiol Chem. 1973 May;354(5):481-6 PMID: 4803487
  18. Eukaryotic ribosomal proteins. Two-dimensional electrophoretic studies.
    FEBS Lett. 1971 Sep 15;17(1):163-167 PMID: 11946021
  19. Ribosomal proteins. Isolation of proteins from 50S ribosomal subunits of Escherichia coli.
    Eur J Biochem. 1971 Nov 11;23(1):12-6 PMID: 4942547
  20. The molecular weights of rat liver ribosomal proteins determined by "three-dimensional" polyacrylamide gel electrophoresis.
    Mol Gen Genet. 1974;134(1):1-6 PMID: 4457752
  21. Studies on proteins of animal ribosomes. XV. Proteins of the small subunit of rat liver ribosomes: isolation, amino acid composition, tryptic peptides and molecular weights.
    Mol Gen Genet. 1973 Nov 22;126(4):349-56 PMID: 4784812
  22. Ribosomal proteins from rabbit reticulocytes: number and molecular weights of proteins from ribosomal subunits.
    J Mol Biol. 1975 Apr 15;93(3):391-404 PMID: 49436
  23. Molecular weight distribution of proteins in rabbit reticulocyte ribosomal subunits.
    J Mol Biol. 1971 Oct 14;61(1):143-56 PMID: 5004354
  24. Studies on proteins of animal ribosomes. XIV. Analysis of phosphorylated rat liver ribosomal proteins by two-dimensional polyacrylamide gel electrophoresis.
    FEBS Lett. 1972 Oct 1;26(1):233-6 PMID: 4636734
  25. The fractionation of high-molecular-weight ribonucleic acid by polyacrylamide-gel electrophoresis.
    Biochem J. 1967 Jan;102(1):251-7 PMID: 5339944
  26. THE PREPARATION OF RIBOSOMAL PROTEIN FROM ESCHERICHIA COLI WITH LITHIUM CHLORIDE AND UREA.
    Biochem Biophys Res Commun. 1965 Feb 17;18:557-62 PMID: 14301460
  27. Molecular weight distribution of ribosomal proteins from several vertebrate species.
    Mol Gen Genet. 1976 Dec 31;142(4):317-31 PMID: 1272245
  28. Structural and functional evidence for a repeated 50S subunit ribosomal protein.
    Nat New Biol. 1973 Mar 14;242(115):47-9 PMID: 4571811
  29. Enumeration of rabbit reticulocyte ribosomal proteins.
    J Mol Biol. 1971 Dec 14;62(2):403-5 PMID: 5138339
  30. The ribosomal proteins of Escherichia coli. I. Purification of the 30S ribosomal proteins.
    Biochemistry. 1969 Jul;8(7):2897-905 PMID: 4897206
  31. Analogies in protein pattern and conformation among ribosomes from different classes of vertebrates.
    Comp Biochem Physiol B. 1971 Dec 15;40(4):1011-27 PMID: 5002919
  32. Isolation of a 5S RNA-protein complex from mammalian ribosomes.
    Proc Natl Acad Sci U S A. 1971 Aug;68(8):1881-5 PMID: 5001943
  33. Ribosomal proteins. 8. Molecular weights of isolated ribosomal proteins of Escherichia coli.
    Proc Natl Acad Sci U S A. 1970 Dec;67(4):1909-13 PMID: 4923120
  34. Two-dimensional polyacrylamide gel electrophoresis: an improved method for ribosomal proteins.
    Anal Biochem. 1974 Jan;57(1):200-10 PMID: 4817496
  35. A modified two-dimensional gel system for the separation and radioautography of microgram amounts of ribosomal proteins.
    Methods Enzymol. 1974;30:526-39 PMID: 4368332
  36. Two-dimensional polyacrylamide gel electrophoresis of eukaryotic ribosomal proteins.
    Methods Enzymol. 1974;30:506-26 PMID: 4855283
  37. Ribosomal proteins. Isolation of the proteins from 30S ribosomal subunits of Escherichia coli.
    Eur J Biochem. 1971 Nov 11;23(1):7-11 PMID: 4942549
  38. 50-S ribosomal proteins. Purification and partial characterization of two acidic proteins, A 1 and A 2, isolated from 50-S ribosomes of Escherichia coli.
    Eur J Biochem. 1972 Jan 31;25(1):5-12 PMID: 4554105
  39. Ribosomal proteins. VII. Two-dimensional polyacrylamide gel electrophoresis for fingerprinting of ribosomal proteins.
    Anal Biochem. 1970 Aug;36(2):401-12 PMID: 4916449
  40. Ribosomal proteins. XII. Number of proteins in small and large ribosomal subunits of Escherichia coli as determined by two-dimensional gel electrophoresis.
    Proc Natl Acad Sci U S A. 1970 Nov;67(3):1276-82 PMID: 4922286
  41. Sensitive separation procedure for Escherichia coli ribosomal proteins and the resolution of high-molecular-weight components.
    Eur J Biochem. 1974 Jun 15;45(2):541-6 PMID: 4605102
  42. Studies on proteins of animal ribosomes. VII. Isolation of proteins from rat liver ribosomes, their molecular weights, amino acid compositions and secondary structure.
    Mol Gen Genet. 1971;111(3):224-34 PMID: 5563935
  43. The phosphorylation of liver ribosomal proteins in vivo. Evidence that only a single small subunit protein (S6) is phosphorylated.
    J Biol Chem. 1974 Nov 10;249(21):6917-25 PMID: 4423396
  44. THE SYNTHESIS OF HEMOGLOBIN IN A CELL-FREE SYSTEM.
    Proc Natl Acad Sci U S A. 1958 Oct 15;44(10):1029-35 PMID: 16590302
  45. Differences in size and number of 80 S and 70 S ribosomal proteins by dodecyl sulfate gel electrophoresis.
    J Biol Chem. 1971 Nov 25;246(22):6828-34 PMID: 4108251
Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00271254
Published
1976-12-31
Pages
299-316
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00271254
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