Purification and preliminary characterization of an aggregation-sensitive chemoattractant of Dictyostelium minutum.

Journal of bacteriology ·Vol. 133 ·No. 1 ·1978-01-00 ·Pages 403-5

Kakebeeke PI, Mato JM, Konijn TM

Abstract

Aggregative amoebae of Dictyostelium minutum are not attracted by cyclic AMP; they are sensitive to various attracting sources from which yeast extract was chosen to purify the chemoattractant. A small acrasin-like species-specific molecule which contains glycine and C5H5N5 has been purified 30,000-fold. Several characteristics of this chemotactic molecule, which is inactivated by an enzyme that is not species specific, are described.

MeSH Terms

Chemotaxis Dictyostelium/physiology Fungal Proteins/isolation & purification Glycine Myxomycetes/physiology Yeast, Dried/analysis

Chemicals

Fungal Proteins Glycine

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Kakebeeke P I

Mato J M

Konijn T M

References (4)

4 references, click to expand

An acrasin-like attractant from yeast extract specific for Dictyostelium lacteum.
Dev Biol. 1977 Jun;57(2):450-3 PMID: 326600
Microbiological assay of cyclic 3',5'-AMP.
Experientia. 1970 Apr 15;26(4):367-9 PMID: 4314777
Preliminary characterization of the acrasin of the cellular slime mold Polysphondylium violaceum.
Proc Natl Acad Sci U S A. 1976 Mar;73(3):795-9 PMID: 3781
Determination of the active portion of the folic acid molecule in cellular slime mold chemotaxis.
J Bacteriol. 1975 Apr;122(1):185-91 PMID: 164431

Article Info

Journal

Journal of bacteriology

Abbr.

J Bacteriol

ISSN

0021-9193

DOI

10.1128/jb.133.1.403-405.1978

Published

1978-01-00

Pages

403-5

Language

English

Region

United States

NLM ID

2985120R

PMCID

PMC222023

Subset

External Links

PubMed source DOI: 10.1128/jb.133.1.403-405.1978 PMC Full Text View journal details

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Purification and preliminary characterization of an aggregation-sensitive chemoattractant of Dictyostelium minutum.

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