Home LiteratureArticle Details
PMID: 3781 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Preliminary characterization of the acrasin of the cellular slime mold Polysphondylium violaceum.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 73 ·No. 3 ·1976-03-00 ·Pages 795-9

Wurster B, Pan P, Tyan GG, Bonner JT

Abstract

Some species of cellular slime mold do not respond to cyclic AMP as an acrasin, or chemoattractant. In one such species, Polysphondylium violaceum, we have isolated and purified its acrasin and determined some of its chemical properties, which lead us to believe it is a small molecule of less than 1500 daltons. One possibility is that it might be a peptide. The acrasin specifically attracts the amoebae of P. violaceum and P. pallidum and fails to do so for six species of Dictyostelium tested. We also have evidence for a specific acrasinase that inactivates the Polysphondylium acrasin.

MeSH Terms
Amines/analysis Chemotaxis Hot Temperature Hydrogen-Ion Concentration Isoelectric Point Myxomycetes/analysis,enzymology,physiology Peptide Hydrolases Peptides/physiology Solubility
Chemicals
Amines Peptides Peptide Hydrolases
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Wurster B
Pan P
Tyan G G
Bonner J T
References (20)
20 references, click to expand
  1. Determination of the active portion of the folic acid molecule in cellular slime mold chemotaxis.
    J Bacteriol. 1975 Apr;122(1):185-91 PMID: 164431
  2. The isolation and partial characterization of neutrophil chemotactic factors from Escherichia coli.
    J Immunol. 1975 Jun;114(6):1831-7 PMID: 165239
  3. Cyclic 3',5'-adenosine monophosphate phosphodiesterase produced by the slime mold Dictyostelium discoideum.
    Science. 1968 Jul 5;161(3836):57-9 PMID: 4298142
  4. Acrasin, Acrasinase, and the sensitivity to acrasin in Dictyostelium discoideum.
    Dev Biol. 1969 Jul;20(1):72-87 PMID: 4307638
  5. Evidence for a second chemotactic system in the cellular slime mold, Dictyostelium discoideum.
    J Bacteriol. 1970 Jun;102(3):682-7 PMID: 4317101
  6. Regulation of extracellular cyclic-AMP-phosphodiesterase activity during development of Dictyostelium discoideum.
    Biochem Biophys Res Commun. 1971 Jan 8;42(1):119-24 PMID: 4322968
  7. Phosphodiesterase in Dictyostelium discoideum and the chemotactic response to cyclic adenosine monophosphate.
    Science. 1972 Mar 3;175(4025):1014-5 PMID: 4333170
  8. Cyclic AMP phosphodiesterase and its inhibitor in slime mould development.
    Nat New Biol. 1972 Jan 19;235(55):90-2 PMID: 4334254
  9. Membrane-bound cyclic AMP phosphodiesterase in chemotactically responding cells of Dictyostelium discoideum.
    Eur J Biochem. 1972 Jun 23;28(1):136-42 PMID: 4340476
  10. Synthesis of cyclic AMP and phosphodiesterase in various species of cellular slime molds and its bearing on chemotaxis and differentiation.
    Dev Biol. 1972 Dec;29(4):402-9 PMID: 4347961
  11. Adenosine 3':5'-cyclic monophosphate concentrations and phosphodiesterase activities during axenic growth and differentiation of cells of the cellular slime mould Dictyostelium discoideum.
    Biochem J. 1973 May;134(1):311-9 PMID: 4353085
  12. Defective cyclic adenosine-3', 5'-phosphate-phosphodiesterase regulation in morphogenetic mutants of Dictyostelium discoideum.
    J Mol Biol. 1973 Mar 15;74(4):573-85 PMID: 4354075
  13. Folic acid as second chemotactic substance in the cellular slime moulds.
    Nat New Biol. 1972 Jun 7;237(75):181-2 PMID: 4504203
  14. The acrasin activity of adenosine-3',5'-cyclic phosphate.
    Proc Natl Acad Sci U S A. 1967 Sep;58(3):1152-4 PMID: 4861307
  15. Determination of the anomeric configuration of 9-alpha-D-mannofuranosyladenine and preparation of 9-alpha-D-lyxofuranosyladenine.
    J Org Chem. 1967 Dec;32(12):4076-8 PMID: 5622475
  16. Studies on proteolytic enzymes (pronase) of Streptomyces griseus K-1. II. Separation of exo- and endopeptidases of pronase.
    J Biochem. 1968 Oct;64(4):427-37 PMID: 5707831
  17. A new approach to the problem of aggregation in the cellular slime molds.
    Biol Bull. 1966 Feb;130(1):28-42 PMID: 5907301
  18. Fractionation of acrasin, a specific chemotactic agent for slime mold aggregation.
    Science. 1956 Jun 29;123(3209):1171-2 PMID: 13337335
  19. Properties of acrasin.
    Science. 1956 Jun 29;123(3209):1172-3 PMID: 13337336
  20. A 5'-Adenosine Monophosphate-Dependent Adenylate Cyclase and an Adenosine 3':5'-Cyclic Monophosphate-Dependent Adenosine Triphosphate Pyrophosphohydrolase in Dictyostelium discoideum.
    Proc Natl Acad Sci U S A. 1973 Apr;70(4):1254-7 PMID: 16592080
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.73.3.795
Published
1976-03-00
Pages
795-9
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC336005
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.73.3.795 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2