Investigation of the mechanism of action of oxotremorine on the guinea-pig isolated ileum preparation.

British journal of pharmacology ·Vol. 41 ·No. 1 ·1971-01-00 ·Pages 19-25

Cox B, Hecker SE

Abstract

1. The effects of oxotremorine on the guinea-pig ileum have been investigated to determine whether any component of its action is due to acetylcholine release.2. Log dose-response curves to oxotremorine and acetylcholine were similar and both drugs were competitively antagonized by atropine.3. Mipafox potentiated acetylcholine but not oxotremorine.4. No evidence of an indirect component of the action of oxotremorine was obtained using various pharmacological procedures (tetrodotoxin, morphine, cooling, procaine and hemicholinium-3).5. Oxotremorine had no effect on acetylcholine release from mipafox treated ileum but decreased the release from physostigmine treated ileum.6. It is concluded that oxotremorine acts directly on muscarinic receptors and not by releasing acetylcholine.

MeSH Terms

Acetylcholine/antagonists & inhibitors,metabolism,pharmacology Amides/pharmacology Animals Atropine/pharmacology Cholinesterase Inhibitors/pharmacology Drug Synergism Guinea Pigs Hemicholinium 3/pharmacology Ileum/drug effects,metabolism In Vitro Techniques Morphine/pharmacology Muscle, Smooth/drug effects Phosphoric Acids/pharmacology Procaine/pharmacology Pyrrolidinones/antagonists & inhibitors,pharmacology Receptors, Drug/drug effects Secretory Rate/drug effects Tetrodotoxin/pharmacology

Chemicals

Amides Cholinesterase Inhibitors Phosphoric Acids Pyrrolidinones Receptors, Drug Hemicholinium 3 Tetrodotoxin Procaine Morphine Atropine Acetylcholine

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Cox B

Hecker S E

References (10)

10 references, click to expand

Inhibition of the release of acetylcholine from isolated guinea-pig ileum by crystalline tetrodotoxin.
J Pharmacol Exp Ther. 1966 Dec;154(3):456-62 PMID: 5928246
Atropine-like properties of hemicholinium-3.
Life Sci. 1967 Mar 1;6(5):537-43 PMID: 4382261
Effects of tetrodotoxin on innervated smooth muscle preparations.
Br J Pharmacol Chemother. 1967 Mar;29(3):259-79 PMID: 4382740
The origin of acetylcholine released from guinea-pig intestine and longitudinal muscle strips.
J Physiol. 1968 Jan;194(1):13-33 PMID: 4295753
The interaction of hemicholinium-3 and oxotremorine in isolated organ preparations.
J Pharm Pharmacol. 1970 Feb;22(2):96-100 PMID: 4392263
Some quantitative uses of drug antagonists.
Br J Pharmacol Chemother. 1959 Mar;14(1):48-58 PMID: 13651579
Effect of cooling, local anaesthetic compounds and botulinum toxin on the responses of and the acetylcholine output from the electrically transmurally stimulated isolated guinea-pig ileum.
Br J Pharmacol Chemother. 1962 Aug;19:42-55 PMID: 13904922
The peripheral actions of oxotremorine, a metabolite of tremorine.
J Pharmacol Exp Ther. 1962 Nov;138:249-57 PMID: 14020934
THE SITE OF THE 5-HYDROXYTRYPTAMINE RECEPTOR ON THE INTRAMURAL NERVOUS PLEXUS OF THE GUINEA-PIG ISOLATED ILEUM.
Br J Pharmacol Chemother. 1963 Oct;21:306-22 PMID: 14081661
THE ORIGIN OF THE ACETYLCHOLINE RELEASED SPONTANEOUSLY FROM THE GUINEA-PIG ISOLATED ILEUM.
Br J Pharmacol Chemother. 1963 Dec;21:555-68 PMID: 14110755

Article Info

Journal

British journal of pharmacology

Abbr.

Br J Pharmacol

ISSN

0007-1188

DOI

10.1111/j.1476-5381.1971.tb09931.x

Published

1971-01-00

Pages

19-25

Language

English

Region

England

NLM ID

7502536

PMCID

PMC1702752

Subset

External Links

PubMed source DOI: 10.1111/j.1476-5381.1971.tb09931.x PMC Full Text View journal details

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Investigation of the mechanism of action of oxotremorine on the guinea-pig isolated ileum preparation.

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