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PMID: 5494224 Published · ppublish English Journal Article

The subcellular localization of di- and tri-peptide hydrolase activity in guinea-pig small intestine.

The Biochemical journal ·Vol. 120 ·No. 1 ·1970-11-00 ·Pages 195-203

Peters TJ

Abstract

1. Two different subcellular fractionation techniques were applied to guinea-pig intestinal mucosa and the composition of the brush borders prepared by the two methods were compared. 2. By using a kinetic assay system the subcellular distribution of activity against ten dipeptides and five tripeptides was studied. 3. Only small amounts (5-10%) of activity against dipeptides were found in the brush-border region, the enzymes being concentrated in the cytosol. 4. Significant amounts (10-60%) of activity against tripeptides were found in the brush border with the remainder largely present in the soluble fraction. 5. The relevance of these studies to the localization in vivo and the possible role of peptidases in protein digestion is discussed.

MeSH Terms
Animals Cell Nucleus/enzymology Cytoplasm/enzymology Digestion Dipeptidases/analysis Dipeptides/metabolism Epithelium/enzymology Guinea Pigs Hydrogen-Ion Concentration Intestinal Mucosa/cytology,enzymology Intestine, Small/enzymology Kinetics Lysosomes/enzymology Microsomes/enzymology Mitochondria/enzymology Peptide Hydrolases/analysis,metabolism Peptides/metabolism
Chemicals
Dipeptides Peptides Peptide Hydrolases Dipeptidases
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Peters T J
References (47)
47 references, click to expand
  1. A CONTINUOUS, AUTOMATIC METHOD FOR THE STUDY OF RATE OF HYDROLYSIS OF PEPTIDES AND AMIDES.
    Anal Biochem. 1965 Apr;11:30-41 PMID: 14328643
  2. The active absorption of amino-acids by the intestine.
    J Physiol. 1953 Aug;121(2):255-63 PMID: 13085334
  3. The purification of aklaline phosphatases of animal tissues.
    Biochem J. 1954 Aug;57(4):595-603 PMID: 13198808
  4. THE METAL ION ACTIVATION OF THE ALKALINE BETA-GLYCEROPHOSPHATASE OF RABBIT SMALL INTESTINE.
    Biochem J. 1965 May;95:475-82 PMID: 14340097
  5. A microspectrophotometric method for the determination of cytochrome oxidase.
    J Biol Chem. 1951 Apr;189(2):665-70 PMID: 14832284
  6. LEUCINE AMINOPEPTIDASE IN HUMAN SERUM: COMPARISON OF HYDROLYSIS OF L-LEUCYLGLYCINE AND L-LEUCYL-BETA-NAPHTHYLAMIDE.
    Clin Chim Acta. 1964 Mar;9:259-68 PMID: 14152343
  7. The assay and reaction kinetics of leucine aminopeptidase from swine kidney.
    Biochem J. 1964 Mar;90(3):509-12 PMID: 5833359
  8. Hydrolases in the mucosa of rat small intestine for phenylalanine-containing dipeptides.
    Biochim Biophys Acta. 1969;185(2):409-23 PMID: 5808704
  9. Rat intestinal microvillus membranes. Purification and biochemical characterization.
    Biochem J. 1968 Jan;106(2):381-90 PMID: 4295331
  10. Studies on the organization of the brush border in intestinal epithelial cells. IV. Aminopeptidase activity in microvillus membranes of hamster intestinal brush borders.
    Biochim Biophys Acta. 1967;135(5):959-65 PMID: 4965166
  11. The distribution of peptidases in subcellular fractions from the mucosa of the small intestine of the rat.
    Biochem J. 1963 Jul;88:162-8 PMID: 13974436
  12. SPECIFIC ASSAYS OF SOME PHOSPHATASES IN SUBCELLULAR FRACTIONS OF SMALL INTESTINAL MUCOSA.
    Nature. 1965 Feb 20;205:799-800 PMID: 14292314
  13. The colorimetric determination of leucine aminopeptidase in urine and serum of normal subjects and patients with cancer and other diseases.
    Cancer. 1958 Mar-Apr;11(2):283-91 PMID: 13511347
  14. On the proteolytic enzymes of animal tissues. IX. Calf thymus tripeptidase.
    J Biol Chem. 1951 Jul;191(1):153-9 PMID: 14850455
  15. Structural and functional organization of the brush border of intestinal epithelial cells. 3. Enzymic activities and chemical composition of various fractions of tris-disrupted brush borders.
    Biochim Biophys Acta. 1967 Jul 3;135(3):475-82 PMID: 4227786
  16. HEREDITARY SUCROSE INTOLERANCE: LEVELS OF SUCRASE ACTIVITY IN JEJUNAL MUCOSA.
    Arch Dis Child. 1964 Oct;39:431-43 PMID: 14223655
  17. Improved methods for determination of RNA and DNA.
    Anal Biochem. 1969 Oct 1;31(1):42-50 PMID: 5380711
  18. Studies on uricase. I. Preparation, purification, and properties of a cuproprotein.
    J Biol Chem. 1955 Oct;216(2):625-41 PMID: 13271340
  19. Subcellular distribution of (lysosomal enzymes) in guinea pig small intestine.
    Life Sci. 1966 Aug;5(15):1365-71 PMID: 5968702
  20. The localization of phosphomonoesterase and aminopeptidase in brush borders isolated from intestinal epithelial cells.
    Biochim Biophys Acta. 1962 Apr 9;58:239-43 PMID: 13908566
  21. STUDIES ON THE HISTOCHEMICAL "LEUCINE AMINOPEPTIDASE" REACTION. 3. ON THE DIFFERENT LNA-SPLITTING ENZYMES FROM SPLEEN.
    Z Zellforch Microsk Anat Histochem. 1964 Apr 10;78:484-6 PMID: 14188683
  22. Purification and properties of an aminopeptidase from kidney cellular particulates.
    J Biol Chem. 1953 May;202(1):1-26 PMID: 13061426
  23. LOCATION OF HYDROLYSIS OF CERTAIN DISACCHARIDES AND PEPTIDES IN THE SMALL INTESTINE.
    Nature. 1964 May 23;202:807-9 PMID: 14187631
  24. ELECTROPHORETIC SEPARATION OF LEUCINE AMINOPEPTIDASE AND LEUCYL-BETA-NAPHTHYLAMIDE-SPLITTING ENZYME.
    Clin Chim Acta. 1964 Dec;10:572-3 PMID: 14254114
  25. Studies on the subcellular localization of peptidases in intestinal mucosa.
    Gut. 1968 Dec;9(6):727 PMID: 5717982
  26. [Studies on aminoacid-arylamidases. I. Distribution and isoenzymes of the aminoacid-arylamidase in the human organism].
    Acta Biol Med Ger. 1967;19(6):809-18 PMID: 5592039
  27. The isolation of purified brush borders from rat small intestine.
    FEBS Lett. 1968 Jul;1(1):46-49 PMID: 11945249
  28. Intracellular hydrolysis of dipeptides during intestinal absorption.
    J Physiol. 1960 Jul;152:367-80 PMID: 14426799
  29. STUDIES ON DIPEPTIDASES AND AMINOPEPTIDASES. I. DISTINCTION BETWEEN LEUCINE AMINOPEPTIDASE AND ENZYMES THAT HYDROLYZE L-LEUCYL-BETA-NAPHTHYLAMIDE.
    J Biol Chem. 1963 Nov;238:3611-20 PMID: 14109195
  30. The isolation of enzymically active nuclei from the rat heart and uterus.
    J Cell Biol. 1967 Mar;32(3):766-70 PMID: 4382331
  31. Observations on the intestinal absorption of some oligopeptides of methionine and glycine in the rat.
    Clin Sci. 1969 Dec;37(3):751-64 PMID: 5363570
  32. Absorption of glycine and glycine peptides from the small intestine of the rat.
    Clin Sci. 1968 Dec;35(3):415-24 PMID: 5715441
  33. Proteolytic and peptidase activities of the jejunum and ileum of the rat during postnatal development.
    Biochem J. 1966 Sep;100(3):775-8 PMID: 5969290
  34. Studies on the fractionation of mucosal homogenates from the small intestine.
    Biochem J. 1965 Dec;97(3):629-42 PMID: 4286674
  35. Improvement in the histochemical localization of leucine aminopeptidase with a new substrate, L-leucyl-4-methoxy-2-naphthylamide.
    J Biophys Biochem Cytol. 1960 Apr;7:261-4 PMID: 14425685
  36. Histochemical localization of intestinal disaccharidases: application to peroral biopsy specimens.
    Nature. 1967 Feb 4;213(5075):516-8 PMID: 6032240
  37. Substrate specificity of mammalian intestinal dipeptidases and tripeptidases.
    Biochem J. 1969 Mar;111(5):30P PMID: 5783451
  38. Evolution of the peroxisome.
    Ann N Y Acad Sci. 1969 Dec 19;168(2):369-81 PMID: 5270945
  39. The sites of hydrolysis of dipeptides containing leucine and glycine by rat jejunum in vitro.
    Biochem J. 1969 Oct;114(4):855-61 PMID: 5343798
  40. Action of tripeptidase on dipeptides of appropriate structure.
    J Biol Chem. 1955 May;214(1):209-13 PMID: 14367379
  41. Absorption of proteins.
    Br Med Bull. 1967 Sep;23(3):241-6 PMID: 4864370
  42. Protein measurement with the Folin phenol reagent.
    J Biol Chem. 1951 Nov;193(1):265-75 PMID: 14907713
  43. The digestion and absorption of glycine oligopeptides.
    Gut. 1969 Dec;10(12):1055 PMID: 5366270
  44. [On localization of peptidase and proteolytic enzyme activities in isolated brush borders of the small intestinal mucosa of rat].
    Biochem Z. 1965 Dec 31;343(4):346-53 PMID: 4960610
  45. The sulphatase of ox liver. I. The complex nature of the enzyme.
    Biochem J. 1953 Jan;53(1):12-5 PMID: 13032023
  46. Dual localization of beta-glucuronidase in endoplasmic reticulum and in lysosomes.
    Nature. 1967 Feb 4;213(5075):457-60 PMID: 4166303
  47. THE PARTIAL PURIFICATION OF AN AMINO AICD NAPHTHYLAMIDASE FROM HUMAN LIVER.
    J Biol Chem. 1965 Apr;240:1718-21 PMID: 14285513
Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj1200195
Published
1970-11-00
Pages
195-203
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1179584
Subset
IM
External Links
PubMed source DOI: 10.1042/bj1200195 PMC Full Text View journal details
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