Improvement in the histochemical localization of leucine aminopeptidase with a new substrate, L-leucyl-4-methoxy-2-naphthylamide.

The Journal of biophysical and biochemical cytology ·Vol. 7 ·1960-04-00 ·Pages 261-4

NACHLAS MM, MONIS B, ROSENBATT D, SELIGMAN AM

Abstract

A new method for the histochemical demonstration of leucine aminopeptidase in fresh frozen sections was developed with the substrate L-leucyl-4-methoxy-2-naphthylamide. The superior enzyme localization is due to the more rapid rate of coupling of the hydrolysis product, 4-methoxy-2-naphthylamine as compared to 2-naphthylamine itself, and to the low lipid solubility and high substantivity for protein of the copper chelate of the dye formed on coupling with tetrazotized diorthoanisidine. A comparison of the old and the new method is illustrated, and a description is given of the localization of leucine aminopeptidase in the tissues of the rat and man.

Keywords

NAPHTHALENES/chemistry PROTEASES/chemistry

MeSH Terms

2-Naphthylamine Aminopeptidases Animals Endopeptidases Humans Hydrolysis Leucyl Aminopeptidase Male Naphthalenes/chemistry Peptide Hydrolases/chemistry Rats Solubility

Chemicals

Naphthalenes 2-naphthylamide 4-methoxy-2-naphthylamine 2-Naphthylamine Endopeptidases Peptide Hydrolases Aminopeptidases Leucyl Aminopeptidase

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

NACHLAS M M

MONIS B

ROSENBATT D

SELIGMAN A M

References (11)

11 references, click to expand

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Adv Enzymol Relat Subj Biochem. 1951;12:191-257 PMID: 14885020
Chromogenic leucyl substrates for aminopeptidase and papain.
Proc Soc Exp Biol Med. 1955 Jul;89(3):473-6 PMID: 13254799
The histochemical demonstration of leucine aminopeptidase.
J Histochem Cytochem. 1957 May;5(3):264-78 PMID: 13449313
The colorimetric determination of leucine aminopeptidase in urine and serum of normal subjects and patients with cancer and other diseases.
Cancer. 1958 Mar-Apr;11(2):283-91 PMID: 13511347
The relationship between fixation and techniques for the histochemical localization of hydrolytic enzymes.
J Histochem Cytochem. 1958 Sep;6(5):322-39 PMID: 13587974
Study of leucine aminopeptidase in neoplastic and inflammatory tissues with a new histochemical method.
Cancer. 1959 May-Jun;12(3):601-8 PMID: 13652108
Serum leucine aminopeptidase activity: findings in carcinoma of the pancreas, pregnancy and other disorders.
Am J Med Sci. 1959 Nov;238:598-609 PMID: 13794557
The colorimetric determination of leucine aminopeptidase activity with L-leucyl-beta-naphthylamide hydrochloride.
Arch Biochem Biophys. 1955 Aug;57(2):458-74 PMID: 13259661
Histochemical demonstration of aminopeptidase.
J Histochem Cytochem. 1956 May;4(3):217-26 PMID: 13332234
Leucine aminopeptidase in pregnancy.
Obstet Gynecol. 1959 Oct;14:488-90 PMID: 14446470
Serum leucine aminopeptidase activity in normal pregnancy and in patients with hydatidiform mole.
N Engl J Med. 1959 Oct 8;261:746-8 PMID: 13804235

Article Info

Journal

The Journal of biophysical and biochemical cytology

Abbr.

J Biophys Biochem Cytol

ISSN

0095-9901

DOI

10.1083/jcb.7.2.261

Published

1960-04-00

Pages

261-4

Language

English

Region

United States

NLM ID

17840020R

PMCID

PMC2224813

Subset

External Links

PubMed source DOI: 10.1083/jcb.7.2.261 PMC Full Text

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Improvement in the histochemical localization of leucine aminopeptidase with a new substrate, L-leucyl-4-methoxy-2-naphthylamide.

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