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PMID: 5441371 Published · ppublish English Journal Article

Mitochondrial deoxyribonucleic acid from Tetrahymena pyriformis and its kinetic complexity.

The Biochemical journal ·Vol. 116 ·No. 5 ·1970-03-00 ·Pages 811-7

Flavell RA, Jones IG

Abstract

1. Mitochondrial DNA from Tetrahymena pyriformis strain T has a buoyant density (rho) of 1.685 compared with rho1.688 for whole cell DNA. Mitochondrial preparations from T. pyriformis strain W show an enrichment of a light satellite (rho1.686), although this is not obtained free from nuclear DNA (rho1.692). 2. T. pyriformis mitochondrial DNA renatures rapidly and the kinetics of this process indicate a complexity of approx. 3x10(7) daltons. 3. The base-pairing in the renaturation product is of a precise nature, since the ;melting' temperature (80.5 degrees C) is indistinguishable from that of the native DNA (80.5 degrees C). 4. Centrifugation of mitochondrial DNA in an alkaline caesium chloride density gradient gives two bands, implying the separation of the complementary strands.

MeSH Terms
Centrifugation, Density Gradient Chromatography DNA/isolation & purification Hot Temperature Kinetics Mitochondria/analysis Tetrahymena/analysis
Chemicals
DNA
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Flavell R A
Jones I G
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23 references, click to expand
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Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj1160811
Published
1970-03-00
Pages
811-7
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1185503
Subset
IM
External Links
PubMed source DOI: 10.1042/bj1160811 PMC Full Text View journal details
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